Anticorps Polyclonal de lapin anti-HMGB1

• Phare
• Validé par KD/KO

HMGB1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IF, FC, RIP, IHC, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

238

Avis (2)

N° de cat : 10829-1-AP

Synonymes

high mobility group box 1, High mobility group protein 1, High mobility group protein B1, HMG 1, HMG1, HMG3, HMGB1, SBP 1

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Galerie de données de validation

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  WB analysis of Jurkat cells using 10829-1-AP

  WB result of HMGB1 antibody (10829-1-AP, 1:1500) with si-control and si-HMGB1 transfected Jurkat cells.

• 

  WB analysis using 10829-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10829-1-AP (HMGB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 10829-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 10829-1-AP (HMGB1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 10829-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10829-1-AP (HMGB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 10829-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10829-1-AP (HMGB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10829-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10829-1-AP (HMGB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10829-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10829-1-AP (HMGB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10829-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10829-1-AP (HMGB1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse brain using 10829-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10829-1-AP (HMGB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules C6, cellules Jurkat, cellules NIH/3T3, cellules RAW 264.7, tissu rénal de souris
• Résultats positifs en IHC: tissu cérébral de souris, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 10 publications below
• WB: See 185 publications below
• IHC: See 46 publications below
• IF: See 57 publications below
• IP: See 7 publications below
• FC: See 1 publications below
• CoIP: See 4 publications below
• ChIP: See 2 publications below
• RIP: See 1 publications below

Informations sur le produit

10829-1-AP cible HMGB1 dans les applications de WB, IP, IF, FC, RIP, IHC, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HMGB1 Protéine recombinante Ag1264
• Nom complet: high-mobility group box 1
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 25-30 kDa
• Numéro d’acquisition GenBank: BC003378
• Symbole du gène: HMGB1
• Identification du gène (NCBI): 3146
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The HMG (high mobility group) proteins are nonhistone chromosomal proteins that is present in almost all eukaryotic cells, and it functions to stabilize NUCLEOSOME formation and acts as a transcription-factor-like protein that regulates the expression of several genes[PMID: 18160415]. Once injury, infection or other inflammatory stimuli, activated macrophages, mature dendritic cells and natural killer cells can secret HMGB1, which act as a crucial cytokine[PMID: 20163887]. HMGB1 also involved in V(D)J recombination by acting as a cofactor of the RAG complex, stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS)[PMID: 19360789 ]. Act as a Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. HMGB1 (high mobility group box 1) modulates gene expression in the nucleus, but certain immune cells secrete HMGB1 as an extracellular Alarmin to signal tissue damage.The nuclear HMGB1 relocalizes to the extracellular milieu in senescent human and mouse cells in culture and in vivo, which stimulated cytokine secretion through TLR-4 signaling (23649808).This antibody is a rabbit polyclonal antibody raised against full length HMGB1 of human origin. The monomeric HMGB1 is 29 kDa and dimer is 58 kDa.

Publications

Species	Application	Title
mouse	IF	ACS Nano; Tumor-Acidity and Bioorthogonal Chemistry-Mediated On-Site Size Transformation Clustered Nanosystem to Overcome Hypoxic Resistance and Enhance Chemoimmunotherapy.; Authors - Kewei Wang
human,mouse	IHC,IF	ACS Nano; Mitochondria-Targeting Polymer Micelle of Dichloroacetate Induced Pyroptosis to Enhance Osteosarcoma Immunotherapy.; Authors - Jiakang Jin
mouse	WB	ACS Nano; Nanointegrative In Situ Reprogramming of Tumor-Intrinsic Lipid Droplet Biogenesis for Low-Dose Radiation-Activated Ferroptosis Immunotherapy; Authors - Qiqi Zhang
mouse	IHC	Nat Commun; Engineered macrophages as near-infrared light activated drug vectors for chemo-photodynamic therapy of primary and bone metastatic breast cancer.; Authors - Yanjuan Huang
mouse	IF	Small; An NIR-Fluorophore-Based Theranostic for Selective Initiation of Tumor Pyroptosis-Induced Immunotherapy.; Authors - Kewei Wang
human	WB	Nat Commun; VCP recruitment to mitochondria causes mitophagy impairment and neurodegeneration in models of Huntington's disease.; Authors - Xing Guo

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Chun (Verified Customer) (06-19-2022)

This antibody worked well.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HeLa cells

FH

PATRICK (Verified Customer) (12-01-2018)

This antibody worked well. ProteinTech provides antibody specific instructions that were helpful for each of the antibodies we ordered.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: PRIMARY ISOLATED ENDOTHELIAL CELLS

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