Anticorps Polyclonal de lapin anti-GOT1

• Phare
• Validé par KD/KO

GOT1 Polyclonal Antibody for WB, IP, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

36

Avis (1)

N° de cat : 14886-1-AP

Synonymes

AATC, CAT, GIG18, GOT1, Transaminase A

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Galerie de données de validation

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  WB analysis using 14886-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14886-1-AP (GOT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 14886-1-AP

  IP result of anti-GOT1 (IP:14886-1-AP, 4ug; Detection:14886-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IF Staining of L02 using 14886-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed L02 cells using 14886-1-AP (GOT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules L02, cerveau de rat, cerveau de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IF/ICC: cellules L02

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 36 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

14886-1-AP cible GOT1 dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GOT1 Protéine recombinante Ag6671
• Nom complet: glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1)
• Masse moléculaire calculée: 46 kDa
• Poids moléculaire observé: 43-46 kDa
• Numéro d’acquisition GenBank: BC000498
• Symbole du gène: GOT1
• Identification du gène (NCBI): 2805
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Glutamate oxaloacetate transaminase 1 (GOT1) catalyzes the reversible reaction of L-aspartate and alpha-ketoglutarate into oxaloacetate and L-glutamate and plays a key role in carbon and nitrogen metabolism. GOT1 can potentially control the intracellular levels of reactive oxygen species (ROS) through NADPH synthesis and enhances tumor growth. GOT1 expression correlates with the growth of several tumors.

Publications

Species	Application	Title
human	WB	Cell; Systematic discovery of mutation-directed neo-protein-protein interactions in cancer.; Authors - Xiulei Mo
	WB	Cell Metab; Rewiring of Glutamine Metabolism Is a Bioenergetic Adaptation of Human Cells with Mitochondrial DNA Mutations.; Authors - Qiuying Chen
mouse	WB	Cell Res; Glutaminase GLS1 senses glutamine availability in a non-enzymatic manner triggering mitochondrial fusion.; Authors - Wei-Feng Cai ; KD Validated
human	WB	Nat Commun; Sirtuin5 contributes to colorectal carcinogenesis by enhancing glutaminolysis in a deglutarylation-dependent manner.; Authors - Yun-Qian Wang
human	WB	Nat Commun; Cooperative STAT/NF-κB signaling regulates lymphoma metabolic reprogramming and aberrant GOT2 expression.; Authors - Maren Feist
human	WB	J Exp Med; Inhibiting both proline biosynthesis and lipogenesis synergistically suppresses tumor growth.; Authors - Miao Liu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Daniel (Verified Customer) (04-29-2019)

Strong band of the expected size. Weaker non-specific bands.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HK2 and RCC4 (human renal cells)

GOT1 Antibody Western Blot, validation (1:1000 dilution) in HK2 and RCC4 (human renal cells) (Cat no:14886-1-AP)

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