MDH2 Polyclonal antibody

MDH2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15462-1-AP

Synonyms

M MDH, MDH, MDH2, MOR1



Tested Applications

Positive WB detected inHeLa cells, mouse lung tissue, rat lung tissue
Positive IP detected inmouse lung tissue
Positive IHC detected inhuman liver cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 12 publications below

Product Information

15462-1-AP targets MDH2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, Caenorhabditis elegans
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MDH2 fusion protein Ag7750
Full Name malate dehydrogenase 2, NAD (mitochondrial)
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC001917
Gene Symbol MDH2
Gene ID (NCBI) 4191
RRIDAB_2878143
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MDH2 antibody 15462-1-APDownload protocol
IHC protocol for MDH2 antibody 15462-1-APDownload protocol
IP protocol for MDH2 antibody 15462-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.

Authors - Xiao-Hui Wang
humanWB

J Exp Med

Inhibiting both proline biosynthesis and lipogenesis synergistically suppresses tumor growth.

Authors - Miao Liu
mouseWB

Aging Dis

Dietary Salt Disrupts Tricarboxylic Acid Cycle and Induces Tau Hyperphosphorylation and Synapse Dysfunction during Aging

Authors - Minghao Yuan
mouseWB

iScience

The Symbiotic Relationship between the Neural Retina and Retinal Pigment Epithelium Is Supported by Utilizing Differential Metabolic Pathways.

Authors - Tirthankar Sinha
mouseWB

Biochim Biophys Acta Mol Basis Dis

Genome-wide translational reprogramming of genes important for myocyte functions in overload-induced heart failure.

Authors - Qianqian Guo

Mol Cell Biol

LONP1 Is Required for Maturation of a Subset of Mitochondrial Proteins, and Its Loss Elicits an Integrated Stress Response.

Authors - Olga Zurita Rendón

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Süleyman (Verified Customer) (02-06-2022)

It is good antibody but some unspecific bands, but MDH2 can be detected specifically around 34 kDa.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HeLa
MDH2 Antibody Western Blot validation (1:1000 dilution) in HeLa (Cat no:15462-1-AP)