Tested Applications
| Positive WB detected in | HeLa cells, mouse lung tissue, HepG2 cells, rat lung tissue, L02 cells |
| Positive IHC detected in | human liver cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15462-1-AP targets MDH2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, caenorhabditis elegans |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7750 Product name: Recombinant human MDH2 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 1-338 aa of BC001917 Sequence: MLSALARPVSAALRRSFSTSAQNNAKVAVLGASGGIGQPLSLLLKNSPLVSRLTLYDIAHTPGVAADLSHIETKAAVKGYLGPEQLPDCLKGCDVVVIPAGVPRKPGMTRDDLFNTNATIVATLTAACAQHCPEAMICVIANPVNSTIPITAEVFKKHGVYNPNKIFGVTTLDIVRANTFVAELKGLDPARVNVPVIGGHAGKTIIPLISQCTPKVDFPQDQLTALTGRIQEAGTEVVKAKAGAGSATLSMAYAGARFVFSLVDAMNGKEGVVECSFVKSQETECTYFSTPLLLGKKGIEKNLGIGKVSSFEEKMISDAIPELKASIKKGEDFVKTLK Predict reactive species |
| Full Name | malate dehydrogenase 2, NAD (mitochondrial) |
| Calculated Molecular Weight | 36 kDa |
| Observed Molecular Weight | 33 kDa |
| GenBank Accession Number | BC001917 |
| Gene Symbol | MDH2 |
| Gene ID (NCBI) | 4191 |
| RRID | AB_2878143 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P40926 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MDH2 encodes the Krebs cycle enzyme mitochondrial malate dehydrogenase, which catalyzes the reversible oxidation of malate to oxaloacetate and plays a role in the malate-aspartate NADH shuttle. Mutations in MDH2 Cause Developmental and epileptic encephalopathy 51 (PMID: 27989324).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MDH2 antibody 15462-1-AP | Download protocol |
| IHC protocol for MDH2 antibody 15462-1-AP | Download protocol |
| WB protocol for MDH2 antibody 15462-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-MDH2 antibody (Cat# 15462-1-AP) has 41 total citations published in journals including Nature, Cell Metabolism, Molecular Cell, Nature Communications, Science Translational Medicine, Cell Reports, and Science Advances. Associated research fields span cancer biology (lung, colorectal, breast, liver, and hematologic malignancies), metabolic regulation (TCA cycle, fatty acid oxidation, glucose homeostasis), post-translational modifications (succinylation, lactylation, β-hydroxybutyrylation), mitochondrial biology, neurology, and cardiology.
| Species | Application | Title |
|---|---|---|
J Hepatol Nucleolin lactylation contributes to intrahepatic cholangiocarcinoma pathogenesis via RNA splicing regulation of MADD | ||
Cell Metab Metabolic regulation of the glioblastoma stem cell epitranscriptome by malate dehydrogenase 2 | ||
Drug Resist Updat A tumor-derived lactate/ENO1 lactylation feedback loop facilitates osimertinib resistance of lung adenocarcinoma. | ||
Mol Neurodegener Mitovesicles secreted into the extracellular space of brains with mitochondrial dysfunction impair synaptic plasticity | ||
Nat Commun Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk. |
Reviews
What customers say
One user (Süleyman Bozkurt) rated this antibody 4 out of 5 stars in a Western Blot application on HeLa cells at a 1:1000 dilution. The reviewer noted that while the antibody performs well and specifically detects MDH2 at the expected ~34 kDa molecular weight, some non-specific bands were observed. Overall, the feedback is positive with a minor caveat regarding background bands.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Süleyman (Verified Customer) (02-06-2022) | It is good antibody but some unspecific bands, but MDH2 can be detected specifically around 34 kDa.
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