MDH2 Polyclonal antibody

MDH2 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

20

Reviews (1)

Cat no : 15462-1-AP

Synonyms

MDH 2, MDH, EC:1.1.1.37

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Validation Data Gallery

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  WB analysis using 15462-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15462-1-AP (MDH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 15462-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15462-1-AP (MDH2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of rat lung using 15462-1-AP

  rat lung tissue were subjected to SDS PAGE followed by western blot with 15462-1-AP (MDH2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15462-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15462-1-AP (MDH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse lung using 15462-1-AP

  IP result of anti-MDH2 (IP:15462-1-AP, 4ug; Detection:15462-1-AP 1:300) with mouse lung tissue lysate 3600ug.

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  IHC staining of human liver cancer using 15462-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15462-1-AP (MDH2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15462-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15462-1-AP (MDH2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human normal colon using 15462-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 15462-1-AP (MDH2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 15462-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15462-1-AP (MDH2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 15462-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15462-1-AP (MDH2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse lung tissue, rat lung tissue
• Positive IP detected in: mouse lung tissue
• Positive IHC detected in: human liver cancer tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 19 publications below
• IP: See 1 publications below

Product Information

15462-1-AP targets MDH2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, caenorhabditis elegans
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MDH2 fusion protein Ag7750
• Full Name: malate dehydrogenase 2, NAD (mitochondrial)
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC001917
• Gene Symbol: MDH2
• Gene ID (NCBI): 4191
• RRID: AB_2878143
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MDH2 encodes the Krebs cycle enzyme mitochondrial malate dehydrogenase, which catalyzes the reversible oxidation of malate to oxaloacetate and plays a role in the malate-aspartate NADH shuttle. Mutations in MDH2 Cause Developmental and epileptic encephalopathy 51 (PMID: 27989324).

Publications

Species	Application	Title
human	WB	Nat Commun; Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.; Authors - Xiao-Hui Wang
human	WB	J Exp Med; Inhibiting both proline biosynthesis and lipogenesis synergistically suppresses tumor growth.; Authors - Miao Liu
mouse	WB	Aging Dis; Dietary Salt Disrupts Tricarboxylic Acid Cycle and Induces Tau Hyperphosphorylation and Synapse Dysfunction during Aging; Authors - Minghao Yuan
mouse	WB	iScience; The Symbiotic Relationship between the Neural Retina and Retinal Pigment Epithelium Is Supported by Utilizing Differential Metabolic Pathways.; Authors - Tirthankar Sinha
mouse	WB	Biochim Biophys Acta Mol Basis Dis; Genome-wide translational reprogramming of genes important for myocyte functions in overload-induced heart failure.; Authors - Qianqian Guo
		Mol Cell Biol; LONP1 Is Required for Maturation of a Subset of Mitochondrial Proteins, and Its Loss Elicits an Integrated Stress Response.; Authors - Olga Zurita Rendón

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Süleyman (Verified Customer) (02-06-2022)

It is good antibody but some unspecific bands, but MDH2 can be detected specifically around 34 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa

MDH2 Antibody Western Blot validation (1:1000 dilution) in HeLa (Cat no:15462-1-AP)