Validation Data Gallery
|Positive WB detected in||A431 cells, HepG2 cells, mouse heart tissue, mouse spleen tissue, HEK-293 cells, mouse brain, rat brain|
|Positive IHC detected in||human breast cancer tissue, human skin tissue, rat brain tissue, human liver tissue, rat heart tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells|
|Positive FC detected in||HEK-293 cells|
|Western Blot (WB)||WB : 1:2000-1:16000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
14800-1-AP targets GOT2 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||GOT2 fusion protein Ag6514|
|Full Name||glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2)|
|Calculated molecular weight||47 kDa|
|Observed molecular weight||43-45 kDa|
|GenBank accession number||BC000525|
|Gene ID (NCBI)||2806|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
GOT2 encodes the mitochondrial glutamate oxaloacetate transaminase and plays an essential role in the intracellular NAD(H) redox balance. Upregulation of GOT2 has been reported in various cancers, including breast cancer and pancreatic cancer. GOT2 mutation leads to autosomal recessive neurometabolic disorder.
|Product Specific Protocols|
|WB protocol for GOT2 antibody 14800-1-AP||Download protocol|
|IHC protocol for GOT2 antibody 14800-1-AP||Download protocol|
|IF protocol for GOT2 antibody 14800-1-AP||Download protocol|
|FC protocol for GOT2 antibody 14800-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Cell Stem Cell
Aspartate availability limits hematopoietic stem cell function during hematopoietic regeneration.
Rewiring of Glutamine Metabolism Is a Bioenergetic Adaptation of Human Cells with Mitochondrial DNA Mutations.
Malic enzyme 2 connects the Krebs cycle intermediate fumarate to mitochondrial biogenesis.
Glutaminase GLS1 senses glutamine availability in a non-enzymatic manner triggering mitochondrial fusion.
Sirtuin5 contributes to colorectal carcinogenesis by enhancing glutaminolysis in a deglutarylation-dependent manner.
Cooperative STAT/NF-κB signaling regulates lymphoma metabolic reprogramming and aberrant GOT2 expression.