Anticorps Polyclonal de lapin anti-FOXM1

• Phare
• Validé par KD/KO

FOXM1 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, RIP, chIP, ELISA

Conjugaison

Non conjugué

Publications

75

Avis (3)

N° de cat : 13147-1-AP

Synonymes

FKHL16, forkhead box M1, FOXM1, FOXM1B, HFH 11, HFH11, HNF 3, HNF 3/fork head homolog 11, INS 1, M phase phosphoprotein 2, MPHOSPH2, MPP 2, MPP2, PIG29, TGT3, Transcription factor Trident, TRIDENT, WIN

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Galerie de données de validation

• 

  WB analysis of HeLa using 13147-1-AP

  WB result of FOXM1 antibody (13147-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FOXM1 transfected HeLa cells.

• 

  WB analysis using 13147-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13147-1-AP (FOXM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13147-1-AP

  Non-treated HeLa and thymidine or nocodazole treated HeLa cells were subjected to SDS PAGE followed by western blot with 13147-1-AP (FOXM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin antibody as loading control.

• 

  WB analysis of mouse thymus using 13147-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13147-1-AP (FOXM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 13147-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 13147-1-AP (FOXM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 13147-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 13147-1-AP (FOXM1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13147-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13147-1-AP (FOXM1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13147-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13147-1-AP (FOXM1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 13147-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13147-1-AP (FOXM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 13147-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13147-1-AP (FOXM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of L02 using 13147-1-AP

  IP result of anti-FOXM1 (IP:13147-1-AP, 5ug; Detection:13147-1-AP 1:700) with L02 cells lysate 1560ug.

• 

  IHC staining of human pancreas cancer using 13147-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13147-1-AP (FOXM1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 13147-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13147-1-AP (FOXM1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 13147-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13147-1-AP (FOXM1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 13147-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13147-1-AP (FOXM1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HT-29, cellules A431, cellules HEK-293, cellules HeLa, cellules L02, cellules MCF-7, tissu de côlon de souris, tissu de thymus de souris, tissu splénique de souris, tissu testiculaire de souris, tissu testiculaire humain
• Résultats positifs en IP: cellules L02
• Résultats positifs en IHC: tissu de cancer du pancréas humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 12 publications below
• WB: See 61 publications below
• IP: See 1 publications below
• ChIP: See 8 publications below
• RIP: See 1 publications below

Informations sur le produit

13147-1-AP cible FOXM1 dans les applications de WB, IP, RIP, chIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FOXM1 Protéine recombinante Ag3729
• Nom complet: forkhead box M 1
• Masse moléculaire calculée: 83 kDa
• Poids moléculaire observé: 84-110 kDa
• Numéro d’acquisition GenBank: BC006192
• Symbole du gène: FOXM1
• Identification du gène (NCBI): 2305
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Forkhead box M1 (FOXM1), also named as HNF-3, HFH-11 or Trident, belongs to a superfamily of Fox transcription factors. FOXM1 is strongly associated with cell proliferation, it plays a important role in cell cycle progression by regulating both G1/S and G2/M phases, it is also involved in DNA damage repair, angiogenesis, apoptosis and tissue regeneration. Recently, crucial roles of this transcription factor were found in the development and progression of many cancers including colorectal, lung, prostate, liver and breast cancer. FOXM1 levels are significantly higher and associated with tumor grade in most human tumors, rendering it a potential target of cancer diagnosis and therapies. Catalog#13147-1-AP is a rabbit polyclonal antibody raised against C-terminal of human FOXM1. And this antibody can recognize three kinds of FOXM1, FOXM1-pp(~95-110kDa), FOXM1-B(isoform2,~83kDa) and FOXM1A(isoform4, ~90kDa). Sometimes a molecular weight of about 65 kDa can also be observed, which may be a degradation form of FOXM1 (PMID: 26547933).

Publications

Species	Application	Title
mouse	WB	Cancer Discov; Interferon-a potentiates anti-PD-1 efficacy by remodeling glucose metabolism in the hepatocellular carcinoma microenvironment.; Authors - Bo Hu ; KD Validated
human	WB	Nat Commun; K235 acetylation couples with PSPC1 to regulate the m6A demethylation activity of ALKBH5 and tumorigenesis; Authors - Xiao-Lan Zhang
mouse	WB	Acta Pharm Sin B; Constitutive androstane receptor induced-hepatomegaly and liver regeneration is partially via yes-associated protein activation.; Authors - Yue Gao
mouse	WB	Nat Commun; Vagus-macrophage-hepatocyte link promotes post-injury liver regeneration and whole-body survival through hepatic FoxM1 activation.; Authors - Tomohito Izumi
human	WB	Nat Commun; FoxM1 repression during human aging leads to mitotic decline and aneuploidy-driven full senescence.; Authors - Joana Catarina Macedo
human	WB	Nat Commun; circTP63 functions as a ceRNA to promote lung squamous cell carcinoma progression by upregulating FOXM1.; Authors - Zhuoan Cheng ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kausik (Verified Customer) (02-21-2022)

The antibody worked well with immunoprecipitation and ChIP experiments and we had acceptable results with immunoblotting.

• Applications: Western Blot, Immunoprecipitation, ChIP
• Cell Tissue Type: human cancer-derived cell lines

FH

Iram (Verified Customer) (09-17-2020)

FOXM1 antibody giving a very clear bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000 for western
• Cell Tissue Type: Human colon cancer

FH

Terri (Verified Customer) (11-05-2019)

This antibody distinguishes multiple isoforms of FOXM1.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: human mesothelioma and ovarian cell lines

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