Anticorps Polyclonal de lapin anti-FAK

• Phare
• Validé par KD/KO

FAK Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

92

Avis (1)

N° de cat : 12636-1-AP

Synonymes

FADK, FADK 1, FAK, FAK1, Focal adhesion kinase 1, pp125FAK, Protein tyrosine kinase 2, PTK2, PTK2 protein tyrosine kinase 2

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Galerie de données de validation

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  WB analysis using 12636-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12636-1-AP (FAK antibody) at dilution of 1:70000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 12636-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12636-1-AP (FAK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 12636-1-AP

  IP result of anti-FAK (IP:12636-1-AP, 4ug; Detection:12636-1-AP 1:30000) with HeLa cells lysate 1600 ug.

• 

  IHC staining of human gliomas using 12636-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 12636-1-AP (FAK antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human breast cancer using 12636-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12636-1-AP (FAK antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12636-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12636-1-AP (FAK antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 12636-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using FAK antibody (12636-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red).

• 

  IF Staining of HUVEC using 12636-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using FAK antibody (12636-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HEK-293, cellules HeLa, cellules HUVEC, cellules Jurkat, cellules MCF-7, cellules NIH/3T3
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de gliome humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HUVEC, cellules A549

Dilution recommandée

• Western Blot (WB): WB : 1:20000-1:100000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 80 publications below
• IHC: See 3 publications below
• IF: See 12 publications below
• CoIP: See 3 publications below

Informations sur le produit

12636-1-AP cible FAK dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poulet, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FAK Protéine recombinante Ag3331
• Nom complet: PTK2 protein tyrosine kinase 2
• Masse moléculaire calculée: 1052 aa, 119 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: BC028733
• Symbole du gène: FAK
• Identification du gène (NCBI): 5747
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FAK (Focal adhesion kinase 1) is also named as FAK1, FADK, pp125FAK, FAK and belongs to the protein kinase superfamily. It is a critical tyrosine kinase that modulates cell adhesion, migration, proliferation and survival in response to extracellular signals (PMID:19664602). It also acts as a pivotal signal 'integrator', controlling and coordinating cellular responses that include cell migration, survival, proliferation and, epithelial tissue repair after DNA damage (PMID:20966971). This protein has some isoforms produced by alternative promoter usage and alternative splicing, and the range of the molecular weights are 100-120kD and 40-60kD.

Publications

Species	Application	Title
human	WB	Nat Cell Biol; Gain of miR-151 on chromosome 8q24.3 facilitates tumour cell migration and spreading through downregulating RhoGDIA.; Authors - Ding Jie J
human	IF	Small; A Spatiotemporal Controllable Biomimetic Skin for Accelerating Wound Repair; Authors - Yuewei Chen
human	WB	J Exp Clin Cancer Res; ACTN1 promotes HNSCC tumorigenesis and cisplatin resistance by enhancing MYH9-dependent degradation of GSK-3β and integrin β1-mediated phosphorylation of FAK; Authors - Li Cui
mouse	WB	J Hazard Mater; Polystyrene microplastics induced male reproductive toxicity in mice.; Authors - Haibo Jin
human	WB	Cell Rep; Mechanotransduction in response to ECM stiffening impairs cGAS immune signaling in tumor cells; Authors - Yingqi Liu
human	WB	Oncogene; Circadian regulator NR1D2 regulates glioblastoma cell proliferation and motility.; Authors - Min Yu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Nathalie (Verified Customer) (07-12-2022)

Good for ImmunoHistochemistry

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1/50
• Cell Tissue Type: Human Skin

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