Paxillin Polyclonal antibody

Paxillin Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, Canine, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 22172-1-AP

Synonyms

paxillin, PXN

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Validation Data Gallery

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  WB analysis of RAW 264.7 using 22172-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 22172-1-AP (Paxillin antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 22172-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22172-1-AP (Paxillin antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 22172-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22172-1-AP (Paxillin antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human stomach cancer using 22172-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 22172-1-AP (Paxillin antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach cancer using 22172-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 22172-1-AP (Paxillin antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MDCK using 22172-1-AP

  Immunofluorescent analysis of MDCK cells using 22172-1-AP (Paxillin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: RAW 264.7 cells
• Positive IHC detected in: human breast cancer tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MDCK cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IHC: See 1 publications below

Product Information

The immunogen of 22172-1-AP is Paxillin Fusion Protein expressed in E. coli.

• Tested Reactivity: human, Canine, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Paxillin fusion protein Ag17702
• Full Name: paxillin
• Calculated Molecular Weight: 591 aa, 65 kDa
• GenBank Accession Number: BC136787
• Gene Symbol: PXN
• Gene ID (NCBI): 5829
• RRID: AB_2879016
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IHC	Oncogene; KIF26B, a novel oncogene, promotes proliferation and metastasis by activating the VEGF pathway in gastric cancer.; Authors - H Zhang
human	WB	Sci Rep; A novel cystathionine γ-lyase inhibitor, I194496, inhibits the growth and metastasis of human TNBC via downregulating multiple signaling pathways.; Authors - Ya Liu
human	WB	Sci Rep; Interferon-inducible protein, IFIX, has tumor-suppressive effects in oral squamous cell carcinoma; Authors - ShanWang
human	WB	Int J Oncol; Cystathionine‑γ‑lyase promotes the metastasis of breast cancer via the VEGF signaling pathway.; Authors - Lupeng Wang
human	WB	Cell Death Discov; A novel chemical inhibitor suppresses breast cancer cell growth and metastasis through inhibiting HPIP oncoprotein.; Authors - Pengyun Li
human	WB	Cell Signal; Activated CRH receptors inhibit autophagy by repressing conversion of LC3BI to LC3BII.; Authors - Lai Jin