Anticorps Polyclonal de lapin anti-EEF1A1

• Phare
• Validé par KD/KO

EEF1A1 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Conjugaison

Non conjugué

Publications

26

Avis (1)

N° de cat : 11402-1-AP

Synonymes

CCS 3, CCS3, EEF 1, EEF1A, eEF1A 1, EEF1A1, EF 1 alpha 1, EF Tu, EF1A, Elongation factor 1 alpha 1, Elongation factor Tu, GRAF 1EF, HNGC:16303, LENG7, PTI1

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Galerie de données de validation

• 

  WB analysis of HeLa using 11402-1-AP

  WB result of EEF1A1 antibody (11402-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEF1A1 transfected HeLa cells.

• 

  WB analysis using 11402-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11402-1-AP (EEF1A1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11402-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11402-1-AP (EEF1A1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11402-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11402-1-AP (EEF1A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11402-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11402-1-AP (EEF1A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 11402-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 11402-1-AP (EEF1A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 11402-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 11402-1-AP (EEF1A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 11402-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11402-1-AP (EEF1A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 11402-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 11402-1-AP (EEF1A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11402-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11402-1-AP (EEF1A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 11402-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11402-1-AP (EEF1A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 11402-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 11402-1-AP (EEF1A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11402-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11402-1-AP (EEF1A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 11402-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11402-1-AP (EEF1A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11402-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11402-1-AP (EEF1A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 11402-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11402-1-AP (EEF1A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 11402-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11402-1-AP (EEF1A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11402-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EEF1A1 antibody (11402-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 11402-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EEF1A1 antibody (11402-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 11402-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EEF1A1 antibody (11402-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of HeLa using 11402-1-AP

  Immunofluorescent analysis of Hela cells, using EEF1A1 antibody 11402-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules C2C12, cellules HeLa, cellules HepG2, cellules Jurkat, cellules MCF-7, cellules Neuro-2a, cellules SH-SY5Y, tissu cardiaque de souris, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain, tissu de muscle squelettique de souris, tissu hépatique de souris, tissu ovarien de souris, tissu pancréatique de souris
• Résultats positifs en IHC: tissu cérébral de souris, tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 21 publications below
• IHC: See 4 publications below
• IF: See 7 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

11402-1-AP cible EEF1A1 dans les applications de WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EEF1A1 Protéine recombinante Ag1938
• Nom complet: eukaryotic translation elongation factor 1 alpha 1
• Masse moléculaire calculée: 462 aa, 50 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC014224
• Symbole du gène: EEF1A1
• Identification du gène (NCBI): 1915
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EEF1A1, also named as EEF1A, EF1A and LENG7, belongs to the GTP-binding elongation factor family. This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. It is a typical housekeeping gene product required for the maintenance of cell growth and/or survival. In addition, EEF1A1 protects the aminoester bond against hydrolysis until a correct match between the codon on mRNA and the anti-codon on tRNA can be achieved. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human EEF1A1.

Publications

Species	Application	Title
human	WB	Cell; Acetylation of Cytidine in mRNA Promotes Translation Efficiency.; Authors - Daniel Arango
mouse	WB	Cell Metab; High dietary fructose promotes hepatocellular carcinoma progression by enhancing O-GlcNAcylation via microbiota-derived acetate; Authors - Peng Zhou
human	WB	Nat Commun; Immunoproteasome-specific subunit PSMB9 induction is required to regulate cellular proteostasis upon mitochondrial dysfunction; Authors - Minji Kim ; KD Validated
human,mouse	WB,IF	Nat Chem Biol; Gelation of cytoplasmic expanded CAG RNA repeats suppresses global protein synthesis; Authors - Yuyin Pan
mouse	WB	Sci Adv; CGG repeat RNA G-quadruplexes interact with FMRpolyG to cause neuronal dysfunction in fragile X-related tremor/ataxia syndrome.; Authors - Sefan Asamitsu
human	WB,IF	Cancer Res; The Ubiquitin-like Protein FAT10 Stabilizes eEF1A1 Expression to Promote Tumor Proliferation in a Complex Manner.; Authors - Xiuxia Liu ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tom (Verified Customer) (08-25-2020)

This antibody successfully detects EEF1A1 in western blots. There was one specific and strong band at 50 kDa.

• Applications: Western Blot
• Cell Tissue Type: Heart

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