Phospho-SPHK1 (Ser225) Polyclonal antibody

• 

  WB analysis of L02 using 19561-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 19561-1-AP (SPHK1-Phospho-Ser225 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 19561-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 19561-1-AP (SPHK1-Phospho-Ser225 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 19561-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 19561-1-AP (SPHK1-Phospho-Ser225 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• Featured Product
• KD/KO Validated

Phospho-SPHK1 (Ser225) Polyclonal Antibody for WB, ELISA

Cat No. 19561-1-AP

Publications(16)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, ELISA

Synonyms

Phospho-SPHK1 (Ser225), SK 1, sphingosine kinase 1, SPHK, SPHK1, SPHK1 phopho, SPHK1-Phospho-Ser225, SPK, SPK 1

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Tested Applications

• Positive WB detected in: L02 cells, human testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 14 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

19561-1-AP targets Phospho-SPHK1 (Ser225) in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: sphingosine kinase 1
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 45-47 kDa
• GenBank Accession Number: NM_182965
• Gene Symbol: SPHK1
• Gene ID (NCBI): 8877
• RRID: AB_10950399
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NYA1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SPHK1, also named as SPHK and SPK, catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. SPHK1 acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D, L-threo-dihydrosphingosine, N, N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. The antibody is specific to Ser(311)-phospho of SPHK1(isoform2) or Ser(225)-phospho of SPHK1(isoform1).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Cell Death Differ; Cell fate determined by the activation balance between PKR and SPHK1.; Authors - Han Qiao
human	WB	Ecotoxicol Environ Saf; Combined effect of co-exposure to di (2-ethylhexyl) phthalates and 50-Hz magnetic-fields on promoting human amniotic cells proliferation.; Authors - Liangjing Chen
human	WB	Ecotoxicol Environ Saf; A 50-Hz magnetic-field exposure promotes human amniotic cells proliferation via SphK-S1P-S1PR cascade mediated ERK signaling pathway.; Authors - Liangjing Chen
mouse	WB	Biochim Biophys Acta Mol Basis Dis; Involvement of released sphingosine 1-phosphate/sphingosine 1-phosphate receptor axis in skeletal muscle atrophy.; Authors - Federica Pierucci
human,rat	WB,IHC	Phytomedicine; Geniposide alleviates VEGF-induced angiogenesis by inhibiting VEGFR2/PKC/ERK1/2-mediated SphK1 translocation.; Authors - Yan Wang
rat	WB	Phytother Res; Geniposide downregulates the VEGF/SphK1/S1P pathway and alleviates angiogenesis in rheumatoid arthritis in vivo and in vitro.; Authors - Yan Wang