EEF1D Polyclonal antibody

• Featured Product
• KD/KO Validated

EEF1D Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

7

Reviews (1)

Cat no : 10630-1-AP

Synonyms

Antigen NY CO 4, EEF1D, EF 1 delta, EF 1D, EF1D, Elongation factor 1 delta, FP1047

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Validation Data Gallery

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  WB analysis using 10630-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10630-1-AP (EEF1D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10630-1-AP

  IP result of anti-EEF1D (IP:10630-1-AP, 3ug; Detection:10630-1-AP 1:300) with HeLa cells lysate 1200ug.

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  IP experiment of HeLa using 10630-1-AP

  IP result of anti-EEF1D (IP:10630-1-AP, 3ug; Detection:10630-1-AP 1:300) with HeLa cells lysate 1200ug.

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  IHC staining of human pancreas cancer using 10630-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10630-1-AP (EEF1D antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 10630-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10630-1-AP (EEF1D antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 10630-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10630-1-AP (EEF1D antibody) at dilution of 1:100 (under 10x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, U-87 MG cells, U2OS cells, NIH/3T3 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human pancreas cancer tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Product Information

10630-1-AP targets EEF1D in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EEF1D fusion protein Ag0983
• Full Name: eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein)
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC007847
• Gene Symbol: EEF1D
• Gene ID (NCBI): 1936
• RRID: AB_2230993
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EEF1D, also named as EF1D and EF 1 delta, belongs to the EF-1-beta/EF-1-delta family. It is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP. EEF1D is phosphorylated upon DNA damage, probably by ATM or ATR. The calculated molecular weight of EEF1D is a 31 kDa, but the modified protein is about 35-40 kDa. ( PMID: 21936567 )

Publications

Species	Application	Title
human	WB	Cell Chem Biol; A photoaffinity labeling strategy identified EF1A1 as a binding protein of cyclic dinucleotide 2'3'-cGAMP.; Authors - Yingjie Hou
human,mouse	WB	EMBO Rep; Transformation of eEF1Bδ into heat-shock response transcription factor by alternative splicing.; Authors - Kaitsuka Taku T ; KD Validated
human	WB	J Exp Clin Cancer Res; EEF1D overexpression promotes osteosarcoma cell proliferation by facilitating Akt-mTOR and Akt-bad signaling.; Authors - Dong-Dong Cheng ; KD Validated
mouse	WB,CoIP	Front Mol Biosci; Heat Shock-Induced Dephosphorylation of Eukaryotic Elongation Factor 1BδL by Protein Phosphatase 1.; Authors - Taku Kaitsuka
mouse	WB	Int J Biochem Cell Biol; The alteration of protein profile induced by cigarette smoking via oxidative stress in mice epididymis.; Authors - Zhu Zijue Z
rat	WB	Proteomics; Pulmonary pressure reduction attenuates expression of proteins identified by lung proteomic profiling in pulmonary hypertensive rats.; Authors - ?stergaard Louise L

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Wei (Verified Customer) (03-12-2020)

Sharp and strong band for western blot

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart