Tested Applications
| Positive WB detected in | HeLa cells, MCF-7 cells, U-87 MG cells, U2OS cells, NIH/3T3 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human pancreas cancer tissue, human prostate cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10630-1-AP targets EEF1D in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0983 Product name: Recombinant human EEF1D protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 347-647 aa of BC007847 Sequence: SHRPGPRSGLSVSSLRPNRKMATNFLAHEKIWFDKFKYDDAERRFYEQMNGPVAGASRQENGASVILRDIARARENIQKSLAGSSGPGASSGTSGDHGELVVRIASLEVENQSLRGVVQELQQAISKLEARLNVLEKSSPGHRATAPQTQHVSPMRQVEPPAKKPATPAEDDEDDDIDLFGSDNEEEDKEAAQLREERLRQYAEKKAKKPALVAKSSILLDVKPWDDETDMAQLEACVRSIQLDGLVWGASKLVPVGYGIRKLQIQCVVEDDKVGTDLLEEEITKFEEHVQSVDIAAFNKI Predict reactive species |
| Full Name | eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein) |
| Calculated Molecular Weight | 31 kDa |
| Observed Molecular Weight | 35-40 kDa |
| GenBank Accession Number | BC007847 |
| Gene Symbol | EEF1D |
| Gene ID (NCBI) | 1936 |
| RRID | AB_2230993 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P29692 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
EEF1D, also named as EF1D and EF 1 delta, belongs to the EF-1-beta/EF-1-delta family. It is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP. EEF1D is phosphorylated upon DNA damage, probably by ATM or ATR. The calculated molecular weight of EEF1D is a 31 kDa, but the modified protein is about 35-40 kDa. ( PMID: 21936567 )
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for EEF1D antibody 10630-1-AP | Download protocol |
| IP protocol for EEF1D antibody 10630-1-AP | Download protocol |
| WB protocol for EEF1D antibody 10630-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
EEF1D antibody (Cat# 10630-1-AP) has 13 citations across journals including PNAS, EMBO Journal, Cell Chemical Biology, iScience, Journal of Experimental & Clinical Cancer Research, International Journal of Cancer, J Virol, Frontiers in Molecular Biosciences, Life Science Alliance, Proteomics, and International Journal of Biochemistry & Cell Biology. Research fields span oncology (osteosarcoma, colorectal cancer), virology (HCMV, KSHV), autophagy/neuronal signaling, ER biology, heat-shock response, and proteomics.
| Species | Application | Title |
|---|---|---|
J Exp Clin Cancer Res EEF1D overexpression promotes osteosarcoma cell proliferation by facilitating Akt-mTOR and Akt-bad signaling.
| ||
Proc Natl Acad Sci U S A RNA-targeted proteomics identifies YBX1 as critical for efficient HCMV mRNA translation | ||
Cell Chem Biol A photoaffinity labeling strategy identified EF1A1 as a binding protein of cyclic dinucleotide 2'3'-cGAMP. | ||
EMBO J Autophagosomes anchor an AKAP11-dependent regulatory checkpoint that shapes neuronal PKA signaling | ||
EMBO Rep Transformation of eEF1Bδ into heat-shock response transcription factor by alternative splicing.
| ||
Int J Cancer EEF1D stabilized by SRSF9 promotes colorectal cancer via enhancing the proliferation and metastasis |
Reviews
What customers say
The single review for this product is from Wei Feng (March 2020), who used it for Western Blot on mouse heart tissue at lot 00000833. They gave it a 5-star rating and noted that it produced a "sharp and strong band" for Western blot. Overall, the feedback is positive, highlighting clear and robust signal performance in WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Wei (Verified Customer) (03-12-2020) | Sharp and strong band for western blot
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