Anticorps Monoclonal anti-EEA1
EEA1 Monoclonal Antibody for IF, ELISA
Hôte / Isotype
Mouse / IgM
Réactivité testée
Humain
Applications
WB, IF, ELISA
Conjugaison
Non conjugué
CloneNo.
1G8B2
N° de cat : 66218-1-Ig
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en IF | cellules HeLa |
Dilution recommandée
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| WB | See 1 publications below |
| IF | See 2 publications below |
Informations sur le produit
66218-1-Ig cible EEA1 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain
| Réactivité | Humain |
| Réactivité citée | Humain |
| Hôte / Isotype | Mouse / IgM |
| Clonalité | Monoclonal |
| Type | Anticorps |
| Immunogène | Peptide |
| Nom complet | early endosome antigen 1 |
| Masse moléculaire calculée | 162 kDa |
| Numéro d’acquisition GenBank | NM_003566 |
| Symbole du gène | EEA1 |
| Identification du gène (NCBI) | 8411 |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Chromatographie d'affinité thiophilique |
| Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
Early endosome antigen 1 (EEA1) is a marker of early endosomes and has an important role in endosomal trafficking. EEA1 contains coiled-coil regions and an FYVE-type zinc finger which interacts with phosphatidylinositol-3-phosphate. EEA1 functions as a Rab5 effector, mediates endosome docking and, together with SNAREs, leads to membrane fusion.
Protocole
| Product Specific Protocols | |
|---|---|
| IF protocol for EEA1 antibody 66218-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Clin Invest TMEM25 modulates neuronal excitability and NMDA receptor subunit NR2B degradation. | ||
Theranostics Homotrimer cavin1 interacts with caveolin1 to facilitate tumor growth and activate microglia through extracellular vesicles in glioma. | ||
J Virol The Late Domain of Prototype Foamy Virus Gag Facilitates Autophagic Clearance of Stress Granules by Promoting Amphisome Formation. | ||
Eur J Pharmacol SUMO2-mediated SUMOylation of SH3GLB1 promotes ionizing radiation-induced hypertrophic cardiomyopathy through mitophagy activation. |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Azita (Verified Customer) (06-16-2021) | Immunocytochemistry labelling of (4% PFA) fixed SH-SY5Y cell line that was pre-treated by the drug of interest and labelled by EEA1 monoclonal antibody at dilution of 1:50 showed strong labelling of early endosomes.
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