Anticorps Polyclonal de lapin anti-DRD2

• Phare
• Validé par KD/KO

DRD2 Polyclonal Antibody for IF, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

31

Avis (1)

N° de cat : 55084-1-AP

Synonymes

D(2) dopamine receptor, D2DR, D2R, Dopamine D2 receptor, dopamine receptor D2, DRD2

---

Galerie de données de validation

• 

  WB analysis using 55084-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55084-1-AP (DRD2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of SH-SY5Y using 55084-1-AP

  IP Result of anti-DRD2 (IP:55084-1-AP, 4ug; Detection:55084-1-AP 1:300) with SH-SY5Y cells lysate 3000ug.

• 

  IF Staining of mouse brain using 55084-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DRD2 antibody (55084-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 55084-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DRD2 antibody (55084-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules SH-SY5Y, tissu cérébral de souris
• Résultats positifs en IP: cellules SH-SY5Y,
• Résultats positifs en IF: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• IHC: See 5 publications below
• IF: See 8 publications below

Informations sur le produit

55084-1-AP cible DRD2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: dopamine receptor D2
• Masse moléculaire calculée: 51 kDa
• Poids moléculaire observé: 50-60 kDa
• Numéro d’acquisition GenBank: NM_016574
• Symbole du gène: DRD2
• Identification du gène (NCBI): 1813
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DRD2, also named D2DR and D2R, belongs to the G-protein coupled receptor 1 family. It is one of the five types (D1 to D5) of receptors for dopamine. The activity of DRD2 is mediated by G proteins which inhibit adenylyl cyclase. Defects in DRD2 are associated with dystonia type 11 (DYT11). The antibody is specific to DRD2.

Publications

Species	Application	Title
human		Cell; PMID: 25594175; Authors - Yiqing Yan ; KD Validated
mouse		Mol Psychiatry; ZFP804A mutant mice display sex-dependent schizophrenia-like behaviors.; Authors - Ying Huang
rat		Int J Mol Sci; Biochemical Neuroadaptations in the Rat Striatal Dopaminergic System after Prolonged Exposure to Methamphetamine Self-Administration; Authors - Subramaniam Jayanthi
mouse		J Neuroimmune Pharmacol; 5-HT4 Receptor is Protective for MPTP-induced Parkinson's Disease Mice Via Altering Gastrointestinal Motility or Gut Microbiota; Authors - Chun Cui
human	IHC	Cancers (Basel); Targeting Dopamine Receptor D2 by Imipridone Suppresses Uterine Serous Cancer Malignant Phenotype.; Authors - Wen Hu
mouse		Cancers (Basel); Targeting Cancer Stem Cells with Differentiation Agents as an Alternative to Genotoxic Chemotherapy for the Treatment of Malignant Testicular Germ Cell Tumors.; Authors - Amanda R Loehr

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Ping (Verified Customer) (06-04-2019)

This antibody is good for both immunoprecipitation and western blot.

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: rat brain tissue

Produits associés

DRD1 Polyclonal antibody

17934-1-AP

DRD5 Polyclonal antibody

20310-1-AP

DAT Polyclonal antibody

22524-1-AP

GNAI1 Polyclonal antibody

12617-1-AP