DRD5 Polyclonal antibody

• Featured Product
• KD/KO Validated

DRD5 Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 20310-1-AP

Synonyms

dopamine receptor D5, Dopamine D5 receptor, DBDR, D1beta dopamine receptor, D(5) dopamine receptor

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Validation Data Gallery

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  WB analysis using 20310-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20310-1-AP (DRD5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20310-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20310-1-AP (DRD5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 20310-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20310-1-AP (DRD5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 20310-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20310-1-AP (DRD5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of SH-SY5Y using 20310-1-AP

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.8 ug DRD5 Polyclonal antibody (20310-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

20310-1-AP targets DRD5 in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DRD5 fusion protein Ag14145
• Full Name: dopamine receptor D5
• Calculated Molecular Weight: 477 aa, 53 kDa
• Observed Molecular Weight: 50-53 kDa
• GenBank Accession Number: BC009748
• Gene Symbol: DRD5
• Gene ID (NCBI): 1816
• RRID: AB_10699880
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DRD5, is a G-protein coupled receptor and involved in diversified physiological and pathological processes. this receptor stimulates adenylyl cyclase activity and is expressed in the limbic area of the mammalian brain(PMID:14699430). Studies have shown that DRD5 is highly expressed in colonic macrophages and DRD5 deficiency exacerbates DSS(dextran sodium sulfate)-induced colitis(PMID:34001860). in addition, DRD5 agonists were potent inhibitors of pituitary tumor growth(PMID:28613975).

Publications

Species	Application	Title
human	WB	Cell; PMID: 25594175; Authors - Yiqing Yan ; KD Validated
mouse	WB	Mol Cell; Dopamine Uses the DRD5-ARRB2-PP2A Signaling Axis to Block the TRAF6-Mediated NF-κB Pathway and Suppress Systemic Inflammation.; Authors - Yuqing Wu ; KO Validated
mouse	IHC	Int J Mol Sci; Gastrointestinal Dopamine in Inflammatory Bowel Diseases: A Systematic Review.; Authors - Magdalena Kurnik-Łucka
human	WB	ACS Chem Neurosci; Development of a PC12 Cell Based Assay for Screening Catechol-O-methyltransferase Inhibitors; Authors - Gongliang Zhang
human	WB,IF	Cell Oncol (Dordr); Activation of dopamine receptor D1 inhibits glioblastoma tumorigenicity by regulating autophagic activity.; Authors - Kang Yang
mouse	WB	J Neurochem; Validation of the Targeted Metabolomic Pathway in the Hippocampus and Comparative Analysis with the Prefrontal Cortex of Social Defeat Model Mice.; Authors - Ke Xu

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