DRD1 Polyclonal antibody

• Featured Product
• KD/KO Validated

DRD1 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

19

Cat no : 17934-1-AP

Synonyms

dopamine receptor D1, Dopamine D1 receptor, DADR, D(1A) dopamine receptor

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Validation Data Gallery

• 

  WB analysis of mouse brain using 17934-1-AP

  37°C incubated mouse brain tissue were subjected to SDS PAGE followed by western blot with 17934-1-AP (DRD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 17934-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17934-1-AP (DRD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17934-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17934-1-AP (DRD1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17934-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17934-1-AP (DRD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 17934-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DRD1 antibody (17934-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 17934-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DRD1 antibody (17934-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: 37°C incubated mouse brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 12 publications below
• IHC: See 2 publications below
• IF: See 6 publications below

Product Information

17934-1-AP targets DRD1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DRD1 fusion protein Ag12366
• Full Name: dopamine receptor D1
• Calculated Molecular Weight: 446 aa, 49 kDa
• Observed Molecular Weight: 50-75 kDa
• GenBank Accession Number: BC074978
• Gene Symbol: DRD1
• Gene ID (NCBI): 1812
• RRID: AB_10598308
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Dopamine is a neurotransmitter that plays a crucial role in physical and mental health, such as cardiovascular, hormonal, renal, and central nervous systems (PMID: 29220802). Five subtypes of mammalian dopamine receptors are grouped into two classes, the D1- and D2-like classes. The D1-like class includes D1 and D5 receptors whereas the D2-like class includes D2, D3, D4 subtypes (PMID: 9457173). Dopamine receptor D1 (DRD1) is the most abundant form of dopamine receptor in the central nervous system. DRD1 stimulates adenylate cyclase, modulates D2 receptor activity, regulates neuron growth and differentiation, and mediates several behavioral responses (PMID: 1977312). DRD1 has a calculated molecular weight of 49 kDa, larger apparent molecular weight of 60-80 kDa may be due to glycosylation (PMID: 1281547; 23821371).

Publications

Species	Application	Title
human	WB	Cell; PMID: 25594175; Authors - Yiqing Yan ; KD Validated
rat		Nat Commun; Non-canonical interplay between glutamatergic NMDA and dopamine receptors shapes synaptogenesis; Authors - Nathan Bénac
rat	WB	EMBO Mol Med; MicroRNA expression profile and functional analysis reveal that miR-382 is a critical novel gene of alcohol addiction.; Authors - Li Jingyuan J
human	IF	Elife; The organic cation transporter 2 regulates dopamine D1 receptor signaling at the Golgi apparatus.; Authors - Natasha M Puri
rat	WB	J Affect Disord; Pramipexole improves depression-like behavior in diabetes mellitus with depression rats by inhibiting NLRP3 inflammasome-mediated neuroinflammation and preventing impaired neuroplasticity; Authors - Ping Li
rat	WB	Int J Mol Sci; Effects of Maternal High-Fructose Diet on Long Non-Coding RNAs and Anxiety-like Behaviors in Offspring; Authors - Yuchen Zou

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