|Positive WB detected in||mouse brain tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||mouse brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse brain tissue|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
22524-1-AP targets DAT in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||DAT fusion protein Ag18297|
|Full Name||solute carrier family 6 (neurotransmitter transporter, dopamine), member 3|
|Calculated molecular weight||620 aa, 68 kDa|
|Observed molecular weight||68 kDa|
|GenBank accession number||BC133003|
|Gene ID (NCBI)||6531|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
DAT, also name as SLC6A3, is dopamine transporter which is a member of the sodium- and chloride- dependent neurotransmitter transporter family. Dopamine(DA) released from neurons is cleared by the DA transporter (DAT). Altered dopaminergic signaling is linked to multiple neuropsychiatric disorders, such as attention deficit hyperactive disorder, mood disorders, schizophrenia, autism and so on (PMID:30755521). 22524-1-AP can detect 70~80kDa band, which is consistent with the report (PMID:12746456).
|Product Specific Protocols|
|WB protocol for DAT antibody 22524-1-AP||Download protocol|
|IHC protocol for DAT antibody 22524-1-AP||Download protocol|
|IF protocol for DAT antibody 22524-1-AP||Download protocol|
|IP protocol for DAT antibody 22524-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Reversing a model of Parkinson's disease with in situ converted nigral neurons.
Fine Particulate Matter Triggers α-Synuclein Fibrillization and Parkinson-like Neurodegeneration
J Affect Disord
Dysregulation of striatal dopamine D2/D3 receptor-mediated by hypocretin induces depressive behaviors in rats
Br J Pharmacol
Andrographolide alleviates parkinsonism in MPTP-PD mice via targeting DRP1-mediated mitochondrial fission.
Reconstituting neurovascular unit with primary neural stem cells and brain microvascular endothelial cells in three-dimensional matrix.
Aging (Albany NY)
The loss of dopaminergic neurons in DEC1 deficient mice potentially involves the decrease of PI3K/Akt/GSK3β signaling.