GNAI1 Polyclonal antibody

GNAI1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 12617-1-AP

Synonyms

Gi, GNAI1

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Validation Data Gallery

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  WB analysis using 12617-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12617-1-AP (GNAI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 12617-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 12617-1-AP (GNAI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of SH-SY5Y using 12617-1-AP

  IP result of anti-GNAI1 (IP:12617-1-AP, 4ug; Detection:12617-1-AP 1:1000) with SH-SY5Y cells lysate 800 ug.

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  IHC staining of human kidney using 12617-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12617-1-AP (GNAI1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 12617-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12617-1-AP (GNAI1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 12617-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 12617-1-AP (GNAI1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 12617-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 12617-1-AP (GNAI1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, fetal human brain tissue, rat brain tissue
• Positive IP detected in: SH-SY5Y cells
• Positive IHC detected in: human kidney tissue, rat kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

12617-1-AP targets GNAI1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GNAI1 fusion protein Ag3290
• Full Name: guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1
• Calculated Molecular Weight: 354 aa, 40 kDa
• Observed Molecular Weight: 38-40 kDa
• GenBank Accession Number: BC026326
• Gene Symbol: GNAI1
• Gene ID (NCBI): 2770
• RRID: AB_2111346
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The guanine nucleotide-binding protein G(i), α-1 subunit (GNAI1), also known as Gαi-1, belongs to the Gαi family. GNAI1 is an adenylate cyclase inhibitor that modulates cAMP. GNAI1 exerts inhibitory effects on cAMP production through its interaction with GPCR and the inactivation of adenyl cyclase (PMID: 35678269). GNAI1 has 2 isoforms with molecular weights of 35 and 40 kDa, respectively.

Publications

Species	Application	Title
mouse	WB	Drug Des Devel Ther; Network Pharmacology and Experimental Verification to Explore the Potential Mechanism of Yin-Huo-Tang for Lung Adenocarcinoma Recurrence.; Authors - Dianna Liu
human	WB	J Oncol; Characterization of the Newly Established Homoharringtonine- (HHT-) Resistant Cell Lines and Mechanisms of Resistance; Authors - Fenglin Li