Anticorps Polyclonal de lapin anti-DRP1 (C-terminal)

• Phare
• Validé par KD/KO

DRP1 (C-terminal) Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (6)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

216

Avis (3)

N° de cat : 12957-1-AP

Synonymes

DRP1, Dymple, Dnm1p/Vps1p-like protein, DNM1L,DLP1, DNM1L

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Galerie de données de validation

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  WB analysis using 12957-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12957-1-AP (DRP1 (C-terminal) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12957-1-AP

  WB result of DRP1 (C-terminal) antibody (12957-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DRP1 (C-terminal) transfected HeLa cells.

• 

  WB analysis using 12957-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12957-1-AP (DRP1 (C-terminal) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 12957-1-AP

  IP result of anti-DRP1 (C-terminal) (IP:12957-1-AP, 4ug; Detection:12957-1-AP 1:1000) with mouse brain tissue lysate 2400 ug.

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  IHC staining of mouse cerebellum using 12957-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12957-1-AP (DRP1 (C-terminal) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 12957-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12957-1-AP (DRP1 (C-terminal) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 12957-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DRP1 (C-terminal) antibody (12957-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 12957-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human DRP1 (C-terminal) (12957-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules A549, cellules DU 145, cellules HCT 116, cellules HeLa, cellules HepG2, cellules NIH/3T3, tissu cérébral de rat
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu de cervelet de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 208 publications below
• IHC: See 16 publications below
• IF: See 23 publications below
• IP: See 3 publications below
• CoIP: See 2 publications below

Informations sur le produit

12957-1-AP cible DRP1 (C-terminal) dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Humain, poisson-zèbre, poulet, singe, souris, Hamster, duck
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DRP1 (C-terminal) Protéine recombinante Ag3644
• Nom complet: dynamin 1-like
• Masse moléculaire calculée: 736 aa, 82 kDa
• Poids moléculaire observé: 75-80 kDa
• Numéro d’acquisition GenBank: BC024590
• Symbole du gène: DRP1
• Identification du gène (NCBI): 10059
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DNM1L(Dynamin-1-like protein) , also known as dynamin-related protein 1 (DRP1), belongs to the dynamin family of large GTPases that mediate membrane remodeling during a variety of cellular processes. DNM1L has an important role in the division of growing mitochondria and peroxisomes and also mediates outer mitochondrial membrane fission in mammalian cells. DNM1L is ubiquitously expressed with abundant expression in skeletal muscle, heart, kidney and brain.Variable isoforms of DNM1L have been reported in a tissue-specific manner due to the alternative splicing.

Publications

Species	Application	Title
human	WB	Cell Res; Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression; Authors - Shiqiao Qiu
human	WB,IHC,IP	Signal Transduct Target Ther; Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy; Authors - Yang Meng
human	WB	Cell Metab; Fatty Liver Disease Caused by High-Alcohol-Producing Klebsiella pneumoniae.; Authors - Jing Yuan
mouse	WB	Protein Cell; Senktide blocks aberrant RTN3 interactome to retard memory decline and tau pathology in social isolated Alzheimer's disease mice; Authors - He-Zhou Huang
mouse	WB,IF	Bioact Mater; Profibrogenic macrophage-targeted delivery of mitochondrial protector via exosome formula for alleviating pulmonary fibrosis; Authors - Wei Zhang ; KD Validated
mouse	WB	ACS Cent Sci; Macrophage Inactivation by Small Molecule Wedelolactone via Targeting sEH for the Treatment of LPS-Induced Acute Lung Injury; Authors - Juan Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Mi (Verified Customer) (03-06-2023)

It works pretty well in human white adipocyte cells.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: adipocytes

FH

Samrajni (Verified Customer) (04-27-2022)

The product works well.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: C2C12 cell line

FH

Tom (Verified Customer) (07-07-2021)

Paraformaldehyde fixation and Triton X-100 permeabilization.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: HeLa

DRP1 (C-terminal) Antibody Immunofluorescence validation (1:100 dilution) in HeLa (Cat no:12957-1-AP)

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