Anticorps Polyclonal de lapin anti-DNAJB1

• Phare
• Validé par KD/KO

DNAJB1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications

28

Avis (1)

N° de cat : 13174-1-AP

Synonymes

Heat shock 40 kDa protein 1, hDj-1, Hdj1, hDj 1, DNAJ1

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Galerie de données de validation

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  WB analysis using 13174-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13174-1-AP (DNAJB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13174-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13174-1-AP (DNAJB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 13174-1-AP

  IP result of anti-DNAJB1 (IP:13174-1-AP, 4ug; Detection:13174-1-AP 1:500) with mouse lung tissue lysate 4000ug.

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  IHC staining of mouse testis using 13174-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13174-1-AP (DNAJB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 13174-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 13174-1-AP (DNAJB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 13174-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 13174-1-AP (DNAJB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 13174-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13174-1-AP (DNAJB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 13174-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13174-1-AP (DNAJB1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 13174-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13174-1-AP (DNAJB1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 13174-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DNAJB1 antibody (13174-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of HeLa using 13174-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human DNAJB1 (13174-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules C2C12, cellules C6, tissu hépatique de rat, tissu hépatique de souris, tissu pulmonaire de rat, tissu pulmonaire de souris
• Résultats positifs en IP: tissu pulmonaire de souris
• Résultats positifs en IHC: tissu de cancer de l'œsophage humain, tissu de lymphome humain, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 26 publications below
• IF: See 1 publications below

Informations sur le produit

13174-1-AP cible DNAJB1 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DNAJB1 Protéine recombinante Ag3830
• Nom complet: DnaJ (Hsp40) homolog, subfamily B, member 1
• Masse moléculaire calculée: 340 aa, 40 kDa
• Poids moléculaire observé: 37-40 kDa
• Numéro d’acquisition GenBank: BC019827
• Symbole du gène: DNAJB1
• Identification du gène (NCBI): 3337
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DNAJB1 is a member of the heat shock 40 protein family and has been involved in a variety of cellular processes, including the proteasome pathway, endoplasmic reticulum stress and viral infection. DNAJB1-PRKACA gene fusions have been considered diagnostic for fibrolamellar hepatocellular carcinoma.

Publications

Species	Application	Title
human	WB	Proc Natl Acad Sci U S A; Local translation in nuclear condensate amyloid bodies.; Authors - Phaedra R Theodoridis
mouse	WB	Mol Ther Nucleic Acids; Age-associated changes in microglia and astrocytes ameliorate blood-brain barrier dysfunction; Authors - Jie Pan
human	WB	Int J Mol Sci; PGC1α Promotes Cisplatin Resistance in Ovarian Cancer by Regulating the HSP70/HK2/VDAC1 Signaling Pathway.; Authors - Yanqing Li
mouse	WB	Front Mol Neurosci; Inhibition of Heat Shock Protein 90 by 17-AAG Reduces Inflammation via P2X7 Receptor/NLRP3 Inflammasome Pathway and Increases Neurogenesis After Subarachnoid Hemorrhage in Mice.; Authors - Yuchun Zuo
mouse	WB	J Gerontol A Biol Sci Med Sci; Multifactorial Attenuation of the Murine Heat Shock Response With Age.; Authors - Donald A Jurivich
mouse	WB	Biogerontology; Disrupted HSF1 regulation in normal and exceptional brain aging; Authors - Rachana Trivedi

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Ana (Verified Customer) (03-11-2021)

DNJB1 in mouse samples. 10 ug of total protein. 1:1000 O/N 4ºC incubation in BSA 3% PBST. Secondary ab HRP conjugated 1:5000 1h RT. 2 bands observed.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse Liver & Heart

DNAJB1 Antibody Western Blot validation (1:1000 dilution) in Mouse Liver & Heart (Cat no:13174-1-AP)

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