Hsc70 Monoclonal antibody

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• KD/KO Validated

Hsc70 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

9

CloneNo.

1A4B3

Cat no : 66442-1-Ig

Synonyms

Heat shock 70 kDa protein 8, Heat shock cognate 71 kDa protein, Hsc70, HSC71, HSP71, HSP73, HSP7C, HSPA10, HSPA8

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Validation Data Gallery

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  WB analysis using 66442-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66442-1-Ig (Hsc70 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66442-1-Ig

  Western blot analysis of Hsc70 in various cell lines using 66442-1-Ig (Hsc70 Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66442-1-Ig

  WB result of Hsc70 antibody (66442-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Hsc70 transfected HEK-293 cells.

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  WB analysis of Recombinant protein using 66442-1-Ig

  6*His tagged-recombinant HSP70 (291-641 aa) and HSC70 (346-646 aa) proteins were subjected to SDS PAGE followed by western blot with 66183-1-Ig (HSP70 antibody), 66442-1-Ig (HSC70 antibody), and 66005-1-Ig (His antibody), respectively, at dilution of 1:50000, incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 66442-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66442-1-Ig (Hsc70 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 66442-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66442-1-Ig (Hsc70 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 66442-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using Hsc70 antibody (66442-1-Ig, Clone: 1A4B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: LNCaP cells, HeLa cells, Recombinant protein, HEK-293 cells, Jurkat cells, ROS1728 cells, C6 cells, RAW 264.7 cells, NIH/3T3 cells, HepG2 cells, K-562 cells, HSC-T6 cells, 4T1 cells
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:100000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IF: See 2 publications below

Product Information

66442-1-Ig targets Hsc70 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, goat, monkey, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Hsc70 fusion protein Ag17910
• Full Name: heat shock 70kDa protein 8
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC007276
• Gene Symbol: HSC70
• Gene ID (NCBI): 3312
• RRID: AB_2881812
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HSPA8 (also known as HSC70) is a member of the HSPA (HSP70) family of heat-shock proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. HSPA8 is a constitutively expressed cytosol/nuclear protein able to translocate between cytoplasm and nucleus. Recently it has been reported that HSPA8 can interact with α-synuclein, the critical pathological protein of Parkinson's disease, indicating its implication in neurodegenerative disease. (PMID: 21832061)

Publications

Species	Application	Title
mouse	WB	J Neuroinflammation; p38-TFEB pathways promote microglia activation through inhibiting CMA-mediated NLRP3 degradation in Parkinson's disease.; Authors - Jialong Chen
human,pig	WB,IF	Microbiol Spectr; Heat Shock Protein Member 8 (HSPA8) Is Involved in Porcine Reproductive and Respiratory Syndrome Virus Attachment and Internalization.; Authors - Lei Wang
human	WB	Cell Commun Signal; Discovery of LAMP-2A as potential biomarkers for glioblastoma development by modulating apoptosis through N-CoR degradation.; Authors - Yongjie Wang
human	WB	FEBS Open Bio; A curcumin analogue GO-Y030 depletes cancer stem cells by inhibiting the interaction between the HSP70/HSP40 complex and its substrates; Authors - Maya Suzuki
		Ecotoxicol Environ Saf; PARP1 promotes NLRP3 activation via blocking TFEB-mediated autophagy in rotenone-induced neurodegeneration; Authors - He Zhang
human	IF	J Transl Med; Arginine methylation of HSPA8 by PRMT9 inhibits ferroptosis to accelerate hepatitis B virus-associated hepatocellular carcinoma progression; Authors - Wensheng Deng