HSPA2 Polyclonal antibody

• Featured Product
• KD/KO Validated

HSPA2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 12797-1-AP

Synonyms

HSP70 2, Heat shock-related 70 kDa protein 2, Heat shock protein family A member 2, Heat shock 70 kDa protein 2

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Validation Data Gallery

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  WB analysis using 12797-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12797-1-AP (HSPA2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12797-1-AP

  WB result of HSPA2 antibody (12797-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSPA2 transfected HeLa cells.

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  WB analysis of human skeletal muscle using 12797-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12797-1-AP (HSPA2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 12797-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12797-1-AP (HSPA2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 12797-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12797-1-AP (HSPA2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 12797-1-AP

  IP result of anti-HSPA2 (IP:12797-1-AP, 4ug; Detection:12797-1-AP 1:1000) with mouse skeletal muscle tissue lysate 4000ug.

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  IHC staining of human testis using 12797-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12797-1-AP (HSPA2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 12797-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12797-1-AP (HSPA2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human normal colon using 12797-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12797-1-AP (HSPA2 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate hyperplasia using 12797-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 12797-1-AP (HSPA2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate hyperplasia using 12797-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 12797-1-AP (HSPA2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary tumor using 12797-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12797-1-AP (HSPA2 antibody) at dilution of 1:50 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 12797-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12797-1-AP (HSPA2 antibody) at dilution of 1:50 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 12797-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 12797-1-AP (HSPA2 antibody) at dilution of 1:50 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 12797-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 12797-1-AP (HSPA2 antibody) at dilution of 1:50 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 12797-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSPA2 antibody (12797-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: A431 cells, human brain tissue, human kidney tissue, human skeletal muscle tissue, HeLa cells, Jurkat cells, MCF-7 cells, NIH/3T3 cells, C6 cells, mouse skeletal tissue
• Positive IP detected in: mouse skeletal muscle tissue
• Positive IHC detected in: human normal colon, human ovary tumor tissue, human prostate hyperplasia tissue, human testis tissue, mouse ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:5000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 7 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

12797-1-AP targets HSPA2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HSPA2 fusion protein Ag3539
• Full Name: heat shock 70kDa protein 2
• Calculated Molecular Weight: 639 aa, 70 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC036107
• Gene Symbol: HSPA2
• Gene ID (NCBI): 3306
• RRID: AB_2119687
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human HSPA2 is a member of the HSPA (HSP70) family of heat-shock proteins, encoded by the gene originally described as testis-specific. Recently, it has been reported that HSPA2 can be also expressed in human somatic tissues in a cell-type specific manner. HSPA2 is a cytosol/nuclear protein able to translocate between cytoplasm and nucleus. At physiological temperature, the HSPA2 was localized primarily in cytoplasm whereas, during heat shock, localization shifted to nucleus and nucleoli (PMID: 18452162). HSPA2 is involved in sperm maturity, function and fertility. Aberrant expression of HSPA2 in testes has been connected with male infertility. Recently, HSPA2 has attracted increased interest due to its possible involvement in carcinogenesis of non-testicular tissues. This antibody well recognized the endogenous HSPA2 protein in testis and multiple cell lines. (21373891)

Publications

Species	Application	Title
mouse	IF	Sci Adv; Circular RNAs from BOULE play conserved roles in protection against stress-induced fertility decline.; Authors - Liuze Gao
human	IHC	Cell Death Differ; RNF144A functions as a tumor suppressor in breast cancer through ubiquitin ligase activity-dependent regulation of stability and oncogenic functions of HSPA2.; Authors - Yin-Long Yang ; KD Validated
human	WB	Curr Biol; RPL10L Is Required for Male Meiotic Division by Compensating for RPL10 during Meiotic Sex Chromosome Inactivation in Mice.; Authors - Long Jiang
		J Mol Cell Biol; Cigarette smoke-induced cell cycle arrest in spermatocytes [GC-2spd(ts)] is mediated through cross talk between Ahr-Nrf2 pathway and MAPK signaling.; Authors - Prabagaran Esakky
mouse	WB,IHC	Development; Mutation of Eif4g3, encoding a eukaryotic translation initiation factor, causes male infertility and meiotic arrest of mouse spermatocytes.; Authors - Sun Fengyun F
human	WB,IHC,IF,CoIP	Biochim Biophys Acta Mol Cell Res; The human testis-enriched HSPA2 interacts with HIF-1α in epidermal keratinocytes, yet HIF-1α stability and HIF-1-dependent gene expression rely on the HSPA (HSP70) activity; Authors - Damian Robert Sojka