Anticorps Polyclonal de lapin anti-Dystroglycan

• Phare
• Validé par KD/KO

Dystroglycan Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

11

Avis (2)

N° de cat : 11017-1-AP

Synonymes

DAG1, DAG, Beta DG, Alpha DG Beta dystroglycan, AGRNR

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Galerie de données de validation

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  WB analysis using 11017-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11017-1-AP (Dystroglycan antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 11017-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11017-1-AP (Dystroglycan antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 11017-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11017-1-AP (Dystroglycan antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 11017-1-AP

  IP result of anti-Dystroglycan (IP:11017-1-AP, 4ug; Detection:11017-1-AP 1:500) with mouse brain tissue lysate 2640ug.

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  IHC staining of mouse heart using 11017-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11017-1-AP (Dystroglycan antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 11017-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11017-1-AP (Dystroglycan antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 11017-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 11017-1-AP (Dystroglycan antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 11017-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 11017-1-AP (Dystroglycan antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11017-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11017-1-AP (Dystroglycan antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11017-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11017-1-AP (Dystroglycan antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 11017-1-AP

  Immunofluorescent analysis of HeLa cells using 11017-1-AP (Dystroglycan antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 11017-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Dystroglycan (11017-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HeLa, tissu cérébral de souris, tissu de muscle squelettique de souris
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu cardiaque de souris, tissu cérébral de souris, tissu de muscle squelettique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 4 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

11017-1-AP cible Dystroglycan dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Dystroglycan Protéine recombinante Ag1456
• Nom complet: dystroglycan 1 (dystrophin-associated glycoprotein 1)
• Masse moléculaire calculée: 97 kDa
• Poids moléculaire observé: 40-43 kDa
• Numéro d’acquisition GenBank: BC012740
• Symbole du gène: Dystroglycan
• Identification du gène (NCBI): 1605
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Dystroglycan, also known as DAG1 or DG, was originally isolated from skeletal muscle as an integral membrane component of the dystrophin-glycoprotein complex (DGC). In addition to skeletal muscle, dystroglycan is strongly expressed in heart and smooth muscle, as well as many non-muscle tissues including brain and peripheral nerve (PMID: 12556455). The dystroglycan is involved in a number of processes including laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization. Dystroglycan consists of two subunits (alpha and beta), which are translated from a single mRNA as a propeptide that is proteolytically cleaved into two noncovalently associated proteins (PMID: 16410545). Alpha-dystroglycan is a 156-kDa extracellular peripheral glycoprotein, while beta-dystroglycan is a 43-kDa transmembrane protein (PMID: 9858474). The 43-kDa beta-dystroglycan can be cleaved into a ~30-kDa form (PMID: 14678802; 18458097; 17255331).

Publications

Species	Application	Title
mouse	IHC	Nat Commun; IL-20 antagonist suppresses PD-L1 expression and prolongs survival in pancreatic cancer models.; Authors - Shao-Wei Lu
mouse	WB	Transl Res; AAV-based gene therapy prevents and halts the progression of dilated cardiomyopathy in a mouse model of phosphoglucomutase I deficiency (PGM1-CDG); Authors - B Balakrishnan
human	WB	Mol Oncol; Circular RNA circ-TNRC6B inhibits the proliferation and invasion of esophageal squamous cell carcinoma cells by regulating the miR-452-5p/DAG1 axis; Authors - Ruiyu Xu
human	WB	Cell Commun Signal; Wnt-driven LARGE2 mediates laminin-adhesive O-glycosylation in human colonic epithelial cells and colorectal cancer.; Authors - Vanessa Dietinger
mouse	IHC	Front Cardiovasc Med; Determination of Agrin and Related Proteins Levels as a Function of Age in Human Hearts.; Authors - Katie L Skeffington
human	IHC	J Med Virol; Identification of potential plasma markers for hepatitis B virus-related chronic hepatitis and liver fibrosis/cirrhosis.; Authors - Dandan Zhao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Perrin (Verified Customer) (11-22-2022)

Works very well, with little background

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1 to 100
• Cell Tissue Type: Gut slice, whole mount

FH

Ryan (Verified Customer) (01-10-2018)

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Zebrafish Retina

Dystroglycan Antibody Immunohistochemistry, validation (1:100 dilution) in Zebrafish Retina (Cat no:11017-1-AP)

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