Dystrophin Polyclonal antibody

• Featured Product
• KD/KO Validated

Dystrophin Polyclonal Antibody for WB, IHC, IF, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

27

Reviews (1)

Cat no : 12715-1-AP

Synonyms

DMD, BMD

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Validation Data Gallery

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  WB analysis using 12715-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12715-1-AP (Dystrophin antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12715-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12715-1-AP (Dystrophin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12715-1-AP

  WB result of Dystrophin antibody (12715-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Dystrophin transfected HepG2 cells.

• 

  WB analysis of HepG2 using 12715-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12715-1-AP (Dystrophin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 12715-1-AP

  IP result of anti-Dystrophin (IP:12715-1-AP, 4ug; Detection:12715-1-AP 1:20000) with mouse brain tissue lysate 1280 ug.

• 

  IHC staining of human skeletal muscle using 12715-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 12715-1-AP (Dystrophin antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon using 12715-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12715-1-AP (Dystrophin antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 12715-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12715-1-AP (Dystrophin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 12715-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 12715-1-AP (Dystrophin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 12715-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12715-1-AP (Dystrophin antibody) at dilution of 1:4000 (under 10x lens).

• 

  IHC staining of mouse heart using 12715-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12715-1-AP (Dystrophin antibody) at dilution of 1:4000 (under 40x lens).

• 

  IHC staining of mouse skeletal muscle using 12715-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 12715-1-AP (Dystrophin antibody) at dilution of 1:2000 (under 10x lens).

• 

  IHC staining of mouse skeletal muscle using 12715-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 12715-1-AP (Dystrophin antibody) at dilution of 1:2000 (under 40x lens).

• 

  IHC staining of human heart using 12715-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12715-1-AP (Dystrophin antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 12715-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12715-1-AP (Dystrophin antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of mouse skeletal muscle using 12715-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using 12715-1-AP (Dystrophin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of rat heart using 12715-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat heart tissue using Dystrophin antibody (12715-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Connexin 43 antibody (26980-1-AP, green).

• 

  IF Staining of mouse skeletal muscle using 12715-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using Dystrophin antibody (12715-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse skeletal muscle tissue using 12715-1-AP

  IF result of anti-DMD (12715-1-AP, 1:500) with PFA fixed mouse skeletal muscle tissue by Dr. Daniel Kopinke.

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Tested Applications

• Positive WB detected in: HepG2 cells, mouse brain tissue, mouse lung, rat brain
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse skeletal muscle tissue, human heart tissue, human normal colon, human skeletal muscle tissue, mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse skeletal muscle tissue, rat heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 5 publications below
• IF: See 13 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below

Product Information

12715-1-AP targets Dystrophin in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Dystrophin fusion protein Ag3408
• Full Name: dystrophin
• Calculated Molecular Weight: 3685 aa, 427 kDa
• Observed Molecular Weight: 70 kDa, 430 kDa
• GenBank Accession Number: BC028720
• Gene Symbol: Dystrophin
• Gene ID (NCBI): 1756
• RRID: AB_10640422
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Dystrophin (DMD or BMD) is a large muscle protein whose mutations cause Duchenne muscular dystrophy (DMD) and Becker muscular dystrophy (BMD), the childhood neuromuscular disorders that result in progressive muscle weakness, respiratory difficulties and cardiovascular dysfunction. Dystrophin is a crucial component of the dystrophin-glycoprotein complex which is essential for muscle membrane integrity and stability. Dystrophin is located on the cytoplasmic face of the sarcolemma and connects the cytoskeletal network to the sarcolemma and extracellular matrix. Multiple isoforms of dystrophin exist due to the alternative splicing, with a wide range of MW (69-72, 110-143, 271, 426 kDa). Most tissues contain transcripts of several isoforms.

Publications

Species	Application	Title
mouse	WB	Nat Biomed Eng; Restoration of dystrophin expression in mice by suppressing a nonsense mutation through the incorporation of unnatural amino acids.; Authors - Ningning Shi
mouse	WB,IF	Sci Adv; Semirational bioengineering of AAV vectors with increased potency and specificity for systemic gene therapy of muscle disorders; Authors - Jihad El Andari
mouse	WB,IF	Nat Commun; Identification of genes associated with cortical malformation using a transposon-mediated somatic mutagenesis screen in mice.; Authors - I-Ling Lu ; KD Validated
mouse	IF	Oxid Med Cell Longev; Targeting the Ang2/Tie2 Axis with Tanshinone IIA Elicits Vascular Normalization in Ischemic Injury and Colon Cancer.; Authors - Wei Zou
rat	IHC	Oxid Med Cell Longev; Knockdown of Salusin-β Improves Cardiovascular Function in Myocardial Infarction-Induced Chronic Heart Failure Rats.; Authors - Yu Xu
mouse	IF	Nanomedicine; Muscle cytotoxicity and immuno-reactivity analysis of the porous carbon nanospheres fabricated by high temperature calcination; Authors - JingWen Huang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Lauren (Verified Customer) (08-10-2018)

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: stem cell-derived myotubes

Dystrophin Antibody Immunofluorescence, validation (1:100 dilution) in stem cell-derived myotubes (Cat no:12715-1-AP)

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