RAPSN Polyclonal antibody

RAPSN Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 20453-1-AP

Synonyms

43 kDa postsynaptic protein, CMS1D, CMS1E, RAPSN, RAPsyn, RING finger protein 205, RNF205

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Validation Data Gallery

• 

  WB analysis of U-937 using 20453-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 20453-1-AP (RAPSN Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 20453-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 20453-1-AP (RAPSN Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 20453-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 20453-1-AP (RAPSN Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 20453-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 20453-1-AP (RAPSN Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 20453-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 20453-1-AP (RAPSN Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 20453-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 20453-1-AP (RAPSN Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 20453-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 20453-1-AP (RAPSN Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 20453-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 20453-1-AP (RAPSN Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 20453-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 20453-1-AP (RAPSN Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 20453-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 20453-1-AP (RAPSN Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 20453-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 20453-1-AP (RAPSN Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: U-937 cells, NIH/3T3 cells, Y79 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

The immunogen of 20453-1-AP is RAPSN Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAPSN fusion protein Ag13896
• Full Name: receptor-associated protein of the synapse
• Calculated Molecular Weight: 412 aa, 46 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC004196
• Gene Symbol: RAPSN
• Gene ID (NCBI): 5913
• RRID: AB_2878689
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
	WB	J Appl Physiol (1985); Plasticity changes in neuromuscular junction morphology and related regulatory proteins in the hibernating ground squirrel; Authors - Yue He