Anticorps Polyclonal de lapin anti-CPLX1

CPLX1 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 10246-2-AP

Synonymes

complexin 1, Complexin I, complexin1, CPLX1, CPX I, CPX1, Synaphin 2

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Galerie de données de validation

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  WB analysis using 10246-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10246-2-AP (CPLX1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 10246-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10246-2-AP (CPLX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 10246-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10246-2-AP (CPLX1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using 10246-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CPLX1 antibody (10246-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules SH-SY5Y,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

10246-2-AP cible CPLX1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris, Macaque
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CPLX1 Protéine recombinante Ag0387
• Nom complet: complexin 1
• Masse moléculaire calculée: 15 kDa
• Poids moléculaire observé: 15-20 kDa
• Numéro d’acquisition GenBank: BC002471
• Symbole du gène: CPLX1
• Identification du gène (NCBI): 10815
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Complexin 1 (CPLX1) is a member of the complexin/synaphin gene family, which are cytosolic proteins that function in synaptic vesicle exocytosis. Complexins are enriched in neurons where they colocalize with syntaxin and SNAP-25. Complexins bind weakly to syntaxin alone and not at all to synaptobrevin and SNAP-25, but strongly to the SNAP receptor-core complex composed of these three molecules. Compexins also compete with alpha-SNAP for binding to the core complex but not with other interacting molecules, including synaptotagmin I, and regulate the sequential interactions of alpha-SNAP and synaptotagmins with the SNAP receptor during exocytosis. CLPX1 binds to the SNAP receptor complex and disrupts it, leading to the release of transmitters. Alterations of complexins may contribute to the molecular substrate for abnormalities of neural connectivity in severe mental disorders.

Publications

Species	Application	Title
mouse	WB	Nat Neurosci; The schizophrenia risk gene product miR-137 alters presynaptic plasticity.; Authors - Sandra Siegert
mouse	WB	Mol Ther; MicroRNA137-loaded lipid nanoparticles regulate synaptic proteins in the prefrontal cortex; Authors - Michelle C Palumbo
human	WB	Cell Rep; The complement inhibitor CD59 is required for GABAergic synaptic transmission in the dentate gyrus; Authors - Lang Wen
mouse	WB	J Neurosci; Loss of Protein Arginine Methyltransferase 8 Alters Synapse Composition and Function, Resulting in Behavioral Defects.; Authors - Jay Penney
mouse	WB	Free Radic Biol Med; NOX2 Deficiency Ameliorates Cerebral Injury by Reduction of Complexin II-mediated Glutamate Excitotoxicity in Experimental Stroke.; Authors - Wang Ziying Z
macaque	IHC	J Virol; Acute Simian Varicella Virus Infection Causes Robust and Sustained Changes in Gene Expression in the Sensory Ganglia.; Authors - Nicole Arnold

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