RAB3A Polyclonal antibody

• Featured Product
• KD/KO Validated

RAB3A Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 15029-1-AP

Synonyms

RAB3A, Ras related protein Rab 3A

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Validation Data Gallery

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  WB analysis using 15029-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15029-1-AP (RAB3A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15029-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15029-1-AP (RAB3A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 15029-1-AP

  IP result of anti-RAB3A (IP:15029-1-AP, 4ug; Detection:15029-1-AP 1:1000) with mouse brain tissue lysate 1920 ug.

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  IHC staining of human gliomas using 15029-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15029-1-AP (RAB3A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 15029-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15029-1-AP (RAB3A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 15029-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15029-1-AP (RAB3A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15029-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15029-1-AP (RAB3A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, SH-SY5Y cells, human brain tissue, mouse cerebellum tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human gliomas tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Product Information

15029-1-AP targets RAB3A in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAB3A fusion protein Ag1903
• Full Name: RAB3A, member RAS oncogene family
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC011782
• Gene Symbol: RAB3A
• Gene ID (NCBI): 5864
• RRID: AB_2177378
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Rab3A is a small G-protein of the Rab family, that is implicated in vesicle fusion and regulated secretion, particularly in neurotransmitter release. Rab3 subfamily small G proteins (Rab3A, Rab3B, Rab3C, and Rab3D) control the regulated exocytosis in neuronal/secretory cells. Rab3A is the most abundant Rab GTPase in brain, where it is associated with synaptic vesicles. Rab3A is believed to modulate secretion efficiency by stimulating vesicle recruitment to sites of exocytosis and/ or by recruiting regulatory molecules to the docking/ fusion machinery.

Publications

Species	Application	Title
human	WB,IHC,IF	Mol Oncol; Overexpression of synaptic vesicle protein Rab GTPase 3C promotes vesicular exocytosis and drug resistance in colorectal cancer cells; Authors - Yu-Chan Chang
human	IHC	Mol Cancer; Secretory RAB GTPase 3C modulates IL6-STAT3 pathway to promote colon cancer metastasis and is associated with poor prognosis.; Authors - Yu-Chan Chang
human	WB,IHC	Cell Death Dis; O-GlcNAcylation on Rab3A attenuates its effects on mitochondrial oxidative phosphorylation and metastasis in hepatocellular carcinoma.; Authors - Weicheng Wu ; KD Validated
mouse	WB	FASEB J; Role of RanBP9 on amyloidogenic processing of APP and synaptic protein levels in the mouse brain.; Authors - Lakshmana Madepalli K MK
mouse	WB	PLoS One; RanBP9 Overexpression Accelerates Loss of Pre and Postsynaptic Proteins in the APΔE9 Transgenic Mouse Brain.; Authors - Hongjie Wang
human	WB	Biochem Pharmacol; Zoledronate modulates intracellular vesicle trafficking in mast cells via disturbing the interaction of myosinVa/Rab3a and sytaxin4/VAMP7.; Authors - Shuang Liu