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- KD/KO Validated
RIM1 Polyclonal antibody
RIM1 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 24576-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse brain tissue, rat brain tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 6 publications below |
Product Information
24576-1-AP targets RIM1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | RIM1 fusion protein Ag20161 |
Full Name | regulating synaptic membrane exocytosis 1 |
Calculated Molecular Weight | 1692 aa, 189 kDa |
Observed Molecular Weight | 189 kDa |
GenBank Accession Number | BC152435 |
Gene Symbol | RIMS1 |
Gene ID (NCBI) | 22999 |
RRID | AB_2879618 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RIMS1 is a RAS gene superfamily member that regulates synaptic vesicle exocytosis. RIMS1 also plays a role in the regulation of voltage-gated calcium channels during neurotransmitter and insulin release. Mutations in RIMS1 have suggested a role cognition and have been identified as the cause of cone-rod dystrophy type 7.
Protocols
Product Specific Protocols | |
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WB protocol for RIM1 antibody 24576-1-AP | Download protocol |
IHC protocol for RIM1 antibody 24576-1-AP | Download protocol |
IP protocol for RIM1 antibody 24576-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Neuron Differential Regulation of Evoked and Spontaneous Release by Presynaptic NMDA Receptors
| ||
iScience Using brain cell-type-specific protein interactomes to interpret neurodevelopmental genetic signals in schizophrenia | ||
Neurochem Res Abnormal Expression of FBXL20 in Refractory Epilepsy Patients and a Pilocarpine-Induced Rat Model. | ||
Neurochem Int Effects of exercise-induced fatigue on the morphology of asymmetric synapse and synaptic protein levels in rat striatum. | ||
Exp Neurol Gene knockout of RNA binding motif 5 in the brain alters RIMS2 protein homeostasis in the cerebellum and Hippocampus and exacerbates behavioral deficits after a TBI in mice | ||
Sci Adv Phosphorylation of Doc2 by EphB2 modulates Munc13-mediated SNARE complex assembly and neurotransmitter release |