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  • KD/KO Validated

RIM1 Polyclonal antibody

RIM1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24576-1-AP

Synonyms

CORD7, KIAA0340, Rab 3 interacting molecule 1, Rab 3 interacting protein 2, RAB3IP2, RIM, RIM 1, RIM1, RIMS1



Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24576-1-AP targets RIM1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RIM1 fusion protein Ag20161
Full Name regulating synaptic membrane exocytosis 1
Calculated Molecular Weight 1692 aa, 189 kDa
Observed Molecular Weight 189 kDa
GenBank Accession NumberBC152435
Gene Symbol RIMS1
Gene ID (NCBI) 22999
RRIDAB_2879618
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RIMS1 is a RAS gene superfamily member that regulates synaptic vesicle exocytosis. RIMS1 also plays a role in the regulation of voltage-gated calcium channels during neurotransmitter and insulin release. Mutations in RIMS1 have suggested a role cognition and have been identified as the cause of cone-rod dystrophy type 7.

Protocols

Product Specific Protocols
WB protocol for RIM1 antibody 24576-1-APDownload protocol
IHC protocol for RIM1 antibody 24576-1-APDownload protocol
IP protocol for RIM1 antibody 24576-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Neuron

Differential Regulation of Evoked and Spontaneous Release by Presynaptic NMDA Receptors

Authors -
  • KO Validated
humanWB

iScience

Using brain cell-type-specific protein interactomes to interpret neurodevelopmental genetic signals in schizophrenia

Authors - Yu-Han H Hsu
humanWB

Neurochem Res

Abnormal Expression of FBXL20 in Refractory Epilepsy Patients and a Pilocarpine-Induced Rat Model.

Authors - Pengfei Fu
ratWB

Neurochem Int

Effects of exercise-induced fatigue on the morphology of asymmetric synapse and synaptic protein levels in rat striatum.

Authors - Zhifeng Wang
mouseWB

Exp Neurol

Gene knockout of RNA binding motif 5 in the brain alters RIMS2 protein homeostasis in the cerebellum and Hippocampus and exacerbates behavioral deficits after a TBI in mice

Authors - Kara Snyder
humanWB

Sci Adv

Phosphorylation of Doc2 by EphB2 modulates Munc13-mediated SNARE complex assembly and neurotransmitter release

Authors - Hong Zhang