Anticorps Polyclonal de lapin anti-COTL1

• Phare
• Validé par KD/KO

COTL1 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

9

Avis (1)

N° de cat : 10781-1-AP

Synonymes

CLP, Coactosin like protein, COTL1

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Galerie de données de validation

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  WB analysis using 10781-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10781-1-AP (COTL1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10781-1-AP

  WB result of COTL1 antibody (10781-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-COTL1 transfected HeLa cells.

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  IP experiment of mouse kidney using 10781-1-AP

  IP result of anti-COTL1 (IP:10781-1-AP, 3ug; Detection:10781-1-AP 1:3000) with mouse kidney tissue lysate 12500ug.

• 

  IHC staining of human lung cancer using 10781-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10781-1-AP (COTL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10781-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10781-1-AP (COTL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: tissu rénal de souris
• Résultats positifs en IHC: tissu de cancer du poumon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

10781-1-AP cible COTL1 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: COTL1 Protéine recombinante Ag1230
• Nom complet: coactosin-like 1 (Dictyostelium)
• Masse moléculaire calculée: 16 kDa
• Poids moléculaire observé: 16 kDa
• Numéro d’acquisition GenBank: BC010884
• Symbole du gène: COTL1
• Identification du gène (NCBI): 23406
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

COTL1, also named as CLP, binds to F-actin in a calcium-independent manner. And it has no direct effect on actin depolymerization.

Publications

Species	Application	Title
human	WB,IHC	J Proteome Res; Proteomic analysis of human small cell lung cancer tissues: up-regulation of coactosin-like protein-1.; Authors - Jeong Hye-Cheol HC
human	WB	Oncotarget; Genetic and epigenetic silencing of mircoRNA-506-3p enhances COTL1 oncogene expression to foster non-small lung cancer progression.; Authors - Shanqi Guo
human	IHC	J Inflamm Res; Coactosin-Like Protein in Breast Carcinoma: Friend or Foe?; Authors - Bei Wang
human	WB	PLoS One; TDP-43 identified from a genome wide RNAi screen for SOD1 regulators.; Authors - Somalinga Balajee R BR ; KD Validated
human	WB	Exp Lung Res; Exposure to cigarette smoke extract and lipopolysaccharide modifies cytoskeleton organization in bronchial epithelial cells.; Authors - Claudia D'Anna
human	WB	J Investig Med; Differential expression of PAI-RBP1, C1orf142, and COTL1 in non-small cell lung cancer cell lines with different tumor metastatic potential.; Authors - Sun Wenjing W

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Susanne (Verified Customer) (06-29-2019)

Good staining with low background in WB and IF.For IF fixation was done with methanol 10 min at -20°C (pre cool), the antibody was diluted in 1%BSA/PBS.For WB the antibody was diluted in PBST-0,1%/5%skim milk and incubated over night.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:1000 WB 1:50 IF
• Cell Tissue Type: EBV transformed B cells, CD19+ cells, CD40L/IL-4 stimulated cells

COTL1 Antibody Western Blot,Immunofluorescence, validation (1:1000 WB 1:50 IF dilution) in EBV transformed B cells, CD19+ cells, CD40L/IL-4 stimulated cells (Cat no:10781-1-AP)

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