DICER1 Polyclonal antibody

• Featured Product
• KD/KO Validated

DICER1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

24

Cat no : 20567-1-AP

Synonyms

HERNA, Helicase MOI, Endoribonuclease Dicer, EC:3.1.26.3, DICER

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Validation Data Gallery

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  WB analysis of HepG2 using 20567-1-AP

  WB result of DICER1 antibody (20567-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-DICER1 transfected HepG2 cells.

• 

  WB analysis of HeLa using 20567-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20567-1-AP (DICER1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 20567-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 20567-1-AP (DICER1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 20567-1-AP

  IP result of anti-DICER1 (IP:20567-1-AP, 5ug; Detection:20567-1-AP 1:300) with Jurkat cells lysate 2000ug.

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  IHC staining of human testis using 20567-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20567-1-AP (DICER1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human testis using 20567-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20567-1-AP (DICER1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human lung cancer using 20567-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20567-1-AP (DICER1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 20567-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20567-1-AP (DICER1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 20567-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20567-1-AP (DICER1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 20567-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20567-1-AP (DICER1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of human ovary tumor using 20567-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using DICER1 antibody (20567-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human ovary tumor using 20567-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using DICER1 antibody (20567-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 20567-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DICER1 antibody (20567-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  FC experiment of HepG2 using 20567-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human DICER1 (20567-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HeLa cells, K-562 cells, HepG2 cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human testis tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human ovary tumor tissue
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 22 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Product Information

20567-1-AP targets DICER1 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: dicer 1, ribonuclease type III
• Calculated Molecular Weight: 219 kDa
• Observed Molecular Weight: 220-250 kDa, 90 kDa
• GenBank Accession Number: NM_030621
• Gene Symbol: DICER1
• Gene ID (NCBI): 23405
• RRID: AB_10694287
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DICER1, also named as DICER, HERNA and KIAA0928, belongs to the helicase family and Dicer subfamily. It is required for formation of the RNA induced silencing complex (RISC). DICER1 is a component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, EIF2C2/AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto EIF2C2/AGO2. EIF2C2/AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. DICER1 cleaves double-stranded RNA to produce short interfering RNAs (siRNAs) which target the selective destruction of complementary RNAs. The antibody is specific to DICER1. The calcualted molecular weight of DICER1 is 219 kDa, but modified DICER1 is about 220-250 kDa. DICER1 exists some isoforms with molecular weight 219 kDa and 93 kDa.

Publications

Species	Application	Title
	WB	Neuron; Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.; Authors - Xiaohui Li ; KD Validated
human	WB	Nat Chem Biol; Differential roles of human PUS10 in miRNA processing and tRNA pseudouridylation.; Authors - Jinghui Song
human	WB	Nat Commun; RNA structure probing reveals the structural basis of Dicer binding and cleavage.; Authors - Qing-Jun Luo
human	WB,IP	Nucleic Acids Res; HSP90 and Aha1 modulate microRNA maturation through promoting the folding of Dicer1.; Authors - Xiaochuan Liu
human	WB	Nat Commun; Long non-coding RNA GAS5 controls human embryonic stem cell self-renewal by maintaining NODAL signalling.; Authors - Chen Xu
human	WB	J Pathol; Cancer-associated somatic DICER1 hotspot mutations cause defective miRNA processing and reverse-strand expression bias to predominantly mature 3p strands through loss of 5p strand cleavage.; Authors - Anglesio M S MS ; KO Validated

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