Anticorps Polyclonal de lapin anti-CLTB

• Phare
• Validé par KD/KO

CLTB Polyclonal Antibody for WB, IP, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 10455-1-AP

Synonymes

Clathrin light chain B, clathrin, light chain (Lcb), CLTB, LCB

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Galerie de données de validation

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  WB analysis of mouse thymus using 10455-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10455-1-AP (CLTB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10455-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10455-1-AP (CLTB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10455-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10455-1-AP (CLTB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10455-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10455-1-AP (CLTB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 10455-1-AP

  IP result of anti-CLTB (IP:10455-1-AP, 4ug; Detection:10455-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IF Staining of HeLa using 10455-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CLTB antibody (10455-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HEK-293 using 10455-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using CLTB antibody (10455-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu de thymus de souris, cellules PC-3, tissu cérébral de souris, tissu cérébral humain
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IF/ICC: cellules HeLa, cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

10455-1-AP cible CLTB dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CLTB Protéine recombinante Ag0707
• Nom complet: clathrin, light chain (Lcb)
• Masse moléculaire calculée: 23 kDa
• Poids moléculaire observé: 32-38 kDa
• Numéro d’acquisition GenBank: BC006457
• Symbole du gène: CLTB
• Identification du gène (NCBI): 1212
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. The clathrin molecule has a triskelion shape. Each clathrin triskelion is composed of three identical heavy chains (180 kDa) and three light chains of two types, LCA (CLTA) and LCB (CLTB) (30-40 kDa). The light chain subunits are thought to regulate the formation or disassembly of clathrin coats. (PMID: 2445759; 8374173; 3563513)

Publications

Species	Application	Title
human	WB	Proc Natl Acad Sci U S A; Clathrin light chains' role in selective endocytosis influences antibody isotype switching.; Authors - Shuang Wu ; KD Validated
mouse	WB	Proc Natl Acad Sci U S A; Clathrin light chain diversity regulates membrane deformation in vitro and synaptic vesicle formation in vivo.; Authors - Lisa Redlingshöfer
human	WB	J Virol; Dabie bandavirus Nonstructural Protein Interacts with Actin to Induce F-Actin Rearrangement and Inhibit Viral Adsorption and Entry.; Authors - Hongyun Liu
human	IF	bioRxiv; Downregulation of lysosomal trafficking in ARPE19 cells leads to decreased transfection efficiency at high passage; Authors - Erika M S Hood

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