HIP1R Polyclonal antibody

• Featured Product
• KD/KO Validated

HIP1R Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 16814-1-AP

Synonyms

HIP 12, HIP1 related protein, HIP12, HIP1R, HIP3, ILWEQ, KIAA0655

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Validation Data Gallery

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  WB analysis using 16814-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16814-1-AP (HIP1R antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 16814-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16814-1-AP (HIP1R Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16814-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16814-1-AP (HIP1R Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 16814-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16814-1-AP (HIP1R antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 16814-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16814-1-AP (HIP1R antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 16814-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16814-1-AP (HIP1R antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 16814-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16814-1-AP (HIP1R antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 16814-1-AP

  IP result of anti-HIP1R (IP:16814-1-AP, 4ug; Detection:16814-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human placenta using 16814-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16814-1-AP (HIP1R antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 16814-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16814-1-AP (HIP1R antibody) at dilution of 1:100 (under 10x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, HeLa cells, mouse lung tissue, mouse heart tissue, human liver tissue, mouse kidney tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

16814-1-AP targets HIP1R in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HIP1R fusion protein Ag10353
• Full Name: huntingtin interacting protein 1 related
• Calculated Molecular Weight: 615 aa, 70 kDa, 120 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC067085
• Gene Symbol: HIP1R
• Gene ID (NCBI): 9026
• RRID: AB_2117572
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cell; Targeting Pin1 renders pancreatic cancer eradicable by synergizing with immunochemotherapy.; Authors - Kazuhiro Koikawa ; KD Validated
human	WB	Nat Cell Biol; Acetylation-dependent regulation of PD-L1 nuclear translocation dictates the efficacy of anti-PD-1 immunotherapy.; Authors - Yang Gao ; KO Validated
human	WB	Nat Biomed Eng; Inhibiting PD-L1 palmitoylation enhances T-cell immune responses against tumours.; Authors - Han Yao
human	WB,IP,IF	Nat Chem Biol; HIP1R targets PD-L1 to lysosomal degradation to alter T cell-mediated cytotoxicity.; Authors - Huanbin Wang ; KD Validated
human	WB	Sci Adv; The Huntingtin-interacting protein SETD2/HYPB is an actin lysine methyltransferase.; Authors - Riyad N H Seervai ; KD Validated
human	IHC	J Clin Med; HIP1R Expression and Its Association with PD-1 Pathway Blockade Response in Refractory Advanced NonSmall Cell Lung Cancer: A Gene Set Enrichment Analysis.; Authors - Young Wha Koh