HIP1-Specific Polyclonal antibody

HIP1-Specific Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19688-1-AP

Synonyms

HIP 1, HIP I, HIP1, ILWEQ

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Validation Data Gallery

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  WB analysis of HEK-293 using 19688-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19688-1-AP (HIP1-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 19688-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 19688-1-AP (HIP1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 19688-1-AP

  IP result of anti-HIP1-Specific (IP:19688-1-AP, 4ug; Detection:19688-1-AP 1:1000) with HEK-293 cells lysate 2400ug.

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  IHC staining of human prostate cancer using 19688-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 19688-1-AP (HIP1-Specific antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human prostate cancer using 19688-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 19688-1-AP (HIP1-Specific antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human colon cancer using 19688-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 19688-1-AP (HIP1-Specific antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human colon cancer using 19688-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 19688-1-AP (HIP1-Specific antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse colon tissue, COLO 320 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human prostate cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19688-1-AP targets HIP1-Specific in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: huntingtin interacting protein 1
• Calculated Molecular Weight: 116 kDa
• Observed Molecular Weight: 116 kDa
• GenBank Accession Number: NM_005338
• Gene Symbol: HIP1
• Gene ID (NCBI): 3092
• RRID: AB_10640443
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HIP1 (huntingtin interacting protein-1) is a endocytic protein that plays a role in clathrin-mediated endocytosis and trafficking. HIP1 can interact with huntingtin (HTT) protein whose mutations cause Huntington's disease (HD), thus gets involved in neurodegeneration. By stabilizing receptor tyrosine kinases following ligand-induced endocytosis, HIP1 may act to promote cell survival. HIP1 is overexpressed in a variety of human cancers and may be a potential predictor for cancer progression and prognosis.