Anticorps Polyclonal de lapin anti-CLTA

• Phare
• Validé par KD/KO

CLTA Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

19

Avis (3)

N° de cat : 10852-1-AP

Synonymes

Clathrin light chain A, clathrin, light chain (Lca), CLTA, LCA

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Galerie de données de validation

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  WB analysis of mouse brain using 10852-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10852-1-AP (CLTA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10852-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10852-1-AP (CLTA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 10852-1-AP

  IP result of anti-CLTA (IP:10852-1-AP, 4ug; Detection:10852-1-AP 1:800) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human breast cancer using 10852-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10852-1-AP (CLTA Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10852-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10852-1-AP (CLTA Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 10852-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10852-1-AP (CLTA antibody) at dilution of 1:500 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HEK-293 using 10852-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using 10852-1-AP (CLTA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HEK-293
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 10 publications below
• IP: See 1 publications below

Informations sur le produit

10852-1-AP cible CLTA dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, canin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CLTA Protéine recombinante Ag1299
• Nom complet: clathrin, light chain (Lca)
• Masse moléculaire calculée: 27 kDa
• Poids moléculaire observé: 35-38 kDa
• Numéro d’acquisition GenBank: BC019287
• Symbole du gène: CLTA
• Identification du gène (NCBI): 1211
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. The clathrin molecule has a triskelion shape. Each clathrin triskelion is composed of three identical heavy chains (180 kDa) and three light chains of two types, LCA (CLTA) and LCB (CLTB) (30-40 kDa). The light chain subunits are thought to regulate the formation or disassembly of clathrin coats. (PMID: 2445759; 8374173; 3563513)

Publications

Species	Application	Title
human	WB	Nat Commun; Imaging vesicle formation dynamics supports the flexible model of clathrin-mediated endocytosis.; Authors - Tomasz J Nawara
human	WB	Proc Natl Acad Sci U S A; Clathrin light chains' role in selective endocytosis influences antibody isotype switching.; Authors - Shuang Wu ; KO Validated
human	WB	J Cell Biol; Alternative splicing of clathrin heavy chain contributes to the switch from coated pits to plaques.; Authors - Gilles Moulay
		Small; Persistency of Enlarged Autolysosomes Underscores Nanoparticle-Induced Autophagy in Hepatocytes.; Authors - Ji-Qian Zhang
rat	IF	Acta Pharmacol Sin; GPR97 deficiency ameliorates renal interstitial fibrosis in mouse hypertensive nephropathy; Authors - Ji-Chao Wu
rat	IHC	J Bone Miner Res; Adaptor protein complex 2-mediated, clathrin-dependent endocytosis, and related gene activities, are a prominent feature during maturation stage amelogenesis.; Authors - Lacruz Rodrigo S RS

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Azita (Verified Customer) (06-17-2021)

Immunocytochemistry labelling of (4% PFA) fixed NSC-34 cells by CLTA Polyclonal antibody at dilution of 1:50 showed strong labelling.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: NSC-34 cells

FH

K (Verified Customer) (06-10-2021)

this Ab works well for western blotting in mouse, human and rat cells

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1;1000

FH

Cristian (Verified Customer) (04-17-2019)

Immunofluorescent analysis of (PFA 3%) fixed HeLa cells using 10852-1-AP (CLTA antibody) at dilution of 1:500 and Alexa Fluor 594-conjugated Affinipure Goat Anti-Rabbit IgG (H+L)

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HeLa

CLTA Antibody Immunofluorescence, validation (1:500 dilution) in HeLa (Cat no:10852-1-AP)

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