Alpha Adaptin Polyclonal antibody

Alpha Adaptin Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 11401-1-AP

Synonyms

ADTAA, alpha Adaptin, Alpha adaptin A, Alpha1 adaptin, AP 2 complex subunit alpha 1, AP2 ALPHA, AP2A1, CLAPA1

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Validation Data Gallery

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  WB analysis of HEK-293 using 11401-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11401-1-AP (alpha Adaptin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human spleen using 11401-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 11401-1-AP (alpha Adaptin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 11401-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11401-1-AP (alpha Adaptin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 11401-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11401-1-AP (alpha Adaptin antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HeLa using 11401-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Alpha Adaptin antibody (11401-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, human heart tissue, human spleen tissue
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 8 publications below
• IF: See 2 publications below

Product Information

11401-1-AP targets Alpha Adaptin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Alpha Adaptin fusion protein Ag1927
• Full Name: adaptor-related protein complex 2, alpha 1 subunit
• Calculated Molecular Weight: 977 aa, 108 kDa
• Observed Molecular Weight: 108 kDa
• GenBank Accession Number: BC014214
• Gene Symbol: AP2A1
• Gene ID (NCBI): 160
• RRID: AB_2056316
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AP2A1 encodes adaptor protein complex 2 (AP-2) subunit alpha-1, which is a 100 kDa coated vesicle protein. Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP complexes are involved in the formation of clathrin-coated vesicles (CCVs) by recruiting the scaffold protein, clathrin. AP complexes also play a pivotal role in the cargo selection by recognizing the sorting signals within the cytoplasmic tail of integral membrane proteins. AP-2 is involved in clathrin-dependent endocytosis.

Publications

Species	Application	Title
human	WB	Nat Cell Biol; Molecular identification of a BAR domain-containing coat complex for endosomal recycling of transmembrane proteins.; Authors - Boris Simonetti
		Cell Res; Numb regulates vesicular docking for homotypic fusion of early endosomes via membrane recruitment of Mon1b.; Authors - Ximing Shao
human	WB	Cells; Five Inhibitory Receptors Display Distinct Vesicular Distributions in Murine T Cells; Authors - Jiahe Lu
human	WB	J Immunol; The AP-2 Clathrin Adaptor Mediates Endocytosis of an Inhibitory Killer Cell Ig-like Receptor in Human NK Cells.; Authors - Amanda K Purdy
human	WB	Neurobiol Dis; Sequential screening nominates the Parkinson's disease associated kinase LRRK2 as a regulator of Clathrin-mediated endocytosis.; Authors - George R Heaton
	IF	Inflammopharmacology; Ginsenoside compound-K inhibits the activity of B cells through inducing IgD-B cell receptor endocytosis in mice with collagen-induced arthritis.; Authors - Mei Zhang