Anticorps Polyclonal de lapin anti-Chromogranin A

Chromogranin A Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

N° de cat : 10529-1-AP

Synonymes

CGA, CHGA, Chromogranin A, Pituitary secretory protein I, SP I, Vasostatin I Vasostatin 2



Applications testées

Résultats positifs en WBcellules PC-12,
Résultats positifs en IHCtissu de cancer du poumon humain, tissu de côlon humain, tissu pancréatique humain
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en IF-Ptissu pancréatique de souris, tissu d'intestin grêle de souris

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochimie (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

10529-1-AP cible Chromogranin A dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéerat, Humain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène Chromogranin A Protéine recombinante Ag0807
Nom complet chromogranin A (parathyroid secretory protein 1)
Masse moléculaire calculée 51 kDa
Poids moléculaire observé 70 kDa
Numéro d’acquisition GenBankBC006459
Symbole du gène Chromogranin A
Identification du gène (NCBI) 1113
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Chromogranin A is a member of the granin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells. Chromogranin A is the precursor to several functional peptides including vasostatin, pancreastatin, catestatin and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine). CgA is one of the most used tumor markers in NET's (neuroendocrine tumors) , and elevated CgA concentrations have been demonstrated in serum or plasma of patients with different types of these tumors.

Protocole

Product Specific Protocols
WB protocol for Chromogranin A antibody 10529-1-APDownload protocol
IHC protocol for Chromogranin A antibody 10529-1-APDownload protocol
IF protocol for Chromogranin A antibody 10529-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Cell Metab

Glycerate from intestinal fructose metabolism induces islet cell damage and glucose intolerance.

Authors - Yanru Wu
humanWB

Cell Biosci

Profile of chimeric RNAs and TMPRSS2-ERG e2e4 isoform in neuroendocrine prostate cancer

Authors - Qiong Wang
humanWB

Sci Rep

Generation and characterization of CRISPR/Cas9-mediated MEN1 knockout BON1 cells: a human pancreatic neuroendocrine cell line.

Authors - Azita Monazzam
humanWB

Discov Oncol

Depiction of neuroendocrine features associated with immunotherapy response using a novel one-class predictor in lung adenocarcinoma

Authors - Hao Liu
mouseIHC

Dose Response

The Protective Effects of Apigenin Against Radiation-Induced Intestinal Injury.

Authors - Danjie Liu
humanWB

Bioengineered

A novel hypoxia-driven gene signature that can predict the prognosis of hepatocellular carcinoma.

Authors - Zhirui Zeng

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

balawant (Verified Customer) (07-26-2022)

This Antibody is working great. I have used for colon and colon cancer cell line

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
FH

Susmita (Verified Customer) (04-13-2022)

This antibody works excellent.

  • Applications: Western Blot
  • Primary Antibody Dilution: WB: 1: 1500
  • Cell Tissue Type: PC cell line
FH

James (Verified Customer) (03-31-2021)

Antibody worked well in both recombinant Chromogranin A produced in both HeLa cell lysates and in HEK cells overexpressed with Chromogranin A

  • Applications: Western Blot, Cell culture
  • Cell Tissue Type: HeLa cell lysate IVT expression and HEK cell overexpression
FH

Iram (Verified Customer) (08-14-2020)

Used 1:1000 dilution for immunoblotting. It gives a very clean band.

  • Applications: Western Blot, Immunohistochemistry
  • Primary Antibody Dilution: 1:1000 for western and 1:100 for IHC
  • Cell Tissue Type: Human colon cancer