Anticorps Polyclonal de lapin anti-Chromogranin A
Chromogranin A Polyclonal Antibody for WB, IF, IHC, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, rat, souris
Applications
WB, IHC, IF-P, ELISA
Conjugaison
Non conjugué
N° de cat : 10529-1-AP
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | cellules PC-12, |
Résultats positifs en IHC | tissu de cancer du poumon humain, tissu de côlon humain, tissu pancréatique humain il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
Résultats positifs en IF-P | tissu pancréatique de souris, tissu d'intestin grêle de souris |
Dilution recommandée
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochimie (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
WB | See 5 publications below |
IHC | See 1 publications below |
IF | See 3 publications below |
Informations sur le produit
10529-1-AP cible Chromogranin A dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris
Réactivité | Humain, rat, souris |
Réactivité citée | rat, Humain, souris |
Hôte / Isotype | Lapin / IgG |
Clonalité | Polyclonal |
Type | Anticorps |
Immunogène | Chromogranin A Protéine recombinante Ag0807 |
Nom complet | chromogranin A (parathyroid secretory protein 1) |
Masse moléculaire calculée | 51 kDa |
Poids moléculaire observé | 70 kDa |
Numéro d’acquisition GenBank | BC006459 |
Symbole du gène | Chromogranin A |
Identification du gène (NCBI) | 1113 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par affinité contre l'antigène |
Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
Chromogranin A is a member of the granin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells. Chromogranin A is the precursor to several functional peptides including vasostatin, pancreastatin, catestatin and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine). CgA is one of the most used tumor markers in NET's (neuroendocrine tumors) , and elevated CgA concentrations have been demonstrated in serum or plasma of patients with different types of these tumors.
Protocole
Product Specific Protocols | |
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WB protocol for Chromogranin A antibody 10529-1-AP | Download protocol |
IHC protocol for Chromogranin A antibody 10529-1-AP | Download protocol |
IF protocol for Chromogranin A antibody 10529-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Metab Glycerate from intestinal fructose metabolism induces islet cell damage and glucose intolerance. | ||
Cell Biosci Profile of chimeric RNAs and TMPRSS2-ERG e2e4 isoform in neuroendocrine prostate cancer | ||
Sci Rep Generation and characterization of CRISPR/Cas9-mediated MEN1 knockout BON1 cells: a human pancreatic neuroendocrine cell line. | ||
Discov Oncol Depiction of neuroendocrine features associated with immunotherapy response using a novel one-class predictor in lung adenocarcinoma | ||
Dose Response The Protective Effects of Apigenin Against Radiation-Induced Intestinal Injury. | ||
Bioengineered A novel hypoxia-driven gene signature that can predict the prognosis of hepatocellular carcinoma. |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH balawant (Verified Customer) (07-26-2022) | This Antibody is working great. I have used for colon and colon cancer cell line
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FH Susmita (Verified Customer) (04-13-2022) | This antibody works excellent.
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FH James (Verified Customer) (03-31-2021) | Antibody worked well in both recombinant Chromogranin A produced in both HeLa cell lysates and in HEK cells overexpressed with Chromogranin A
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FH Iram (Verified Customer) (08-14-2020) | Used 1:1000 dilution for immunoblotting. It gives a very clean band.
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