Secretogranin II Polyclonal antibody

Secretogranin II Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, CoIP, ELISA

Conjugate

Unconjugated

Publications

11

Reviews (1)

Cat no : 20357-1-AP

Synonyms

CHGC, Chromogranin C, SCG2, Secretogranin 2, Secretogranin II, SgII, SN

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Validation Data Gallery

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  WB analysis of SH-SY5Y using 20357-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20357-1-AP (Secretogranin II antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse pancreas using 20357-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 20357-1-AP (Secretogranin II antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 20357-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 20357-1-AP (Secretogranin II antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 20357-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 20357-1-AP (Secretogranin II antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 20357-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 20357-1-AP (Secretogranin II antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse pancreas using 20357-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using 20357-1-AP (Secretogranin II antibody), at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: SH-SY5Y cells
• Positive IHC detected in: mouse pancreas tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse pancreas tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 6 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Product Information

20357-1-AP targets Secretogranin II in WB, IHC, IF-P, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Secretogranin II fusion protein Ag14215
• Full Name: secretogranin II (chromogranin C)
• Calculated Molecular Weight: 617 aa, 71 kDa
• Observed Molecular Weight: 71 kDa
• GenBank Accession Number: BC022509
• Gene Symbol: Chromogranin C/SGII
• Gene ID (NCBI): 7857
• RRID: AB_10699881
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SCG2, also known as secretogranin II or Chromogranin C, is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is widely distributed in nervous and endocrine tissues, and abundant in neuroendocrine granules. In the brain secretogranin II is almost completely processed to secretoneurin, a peptide involved in neurite outgrowth, neuroprotection and neuronal plasticity.

Publications

Species	Application	Title
rat	WB	Cell Rep; Gut microbiota shapes social dominance through modulating HDAC2 in the medial prefrontal cortex.; Authors - Tian Wang
mouse,rat	WB,IF	J Cell Biol; SNX5 targets a monoamine transporter to the TGN for assembly into dense core vesicles by AP-3.; Authors - Hongfei Xu
human	WB,IHC,CoIP	Mol Oncol; Secretogranin II impairs tumor growth and angiogenesis by promoting degradation of hypoxia-inducible factor-1α in colorectal cancer.; Authors - Chao Fang
human	IHC	Front Mol Biosci; Tumor Microenvironment Heterogeneity-Based Score System Predicts Clinical Prognosis and Response to Immune Checkpoint Blockade in Multiple Colorectal Cancer Cohorts.; Authors - Hufei Wang
human	IHC	Front Physiol; Potential biomarkers and immune cell infiltration involved in aortic valve calcification identified through integrated bioinformatics analysis; Authors - Xiaoshuo Lv
mouse	WB	Front Neurol; Tandem mass tag-based quantitative proteomic analysis of effects of multiple sevoflurane exposures on the cerebral cortex of neonatal and adult mice; Authors - Jingyu Feng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Wojciech (Verified Customer) (03-02-2023)

The antibody worked very well. The dilution used was 1:1000 in 5% non-fat milk. The single detected band size was around 75 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: T-ALL cell lines