Anticorps Polyclonal de lapin anti-CHAD

CHAD Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 12963-1-AP

Synonymes

Cartilage leucine rich protein, CHAD, chondroadherin, SLRR4A

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Galerie de données de validation

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  WB analysis of HeLa using 12963-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12963-1-AP (CHAD antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 12963-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12963-1-AP (CHAD antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12963-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12963-1-AP (CHAD antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

12963-1-AP cible CHAD dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CHAD Protéine recombinante Ag4077
• Nom complet: chondroadherin
• Masse moléculaire calculée: 359 aa, 40.5 kDa
• Poids moléculaire observé: 41 kDa
• Numéro d’acquisition GenBank: BC036360
• Symbole du gène: CHAD
• Identification du gène (NCBI): 1101
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Chondroadherin (CHAD), also known as cartilage leucine-rich protein, is a prominent noncollagenous extracellular protein in cartilage. It was first isolated from bovine cartilage. The primary structure of CHAD shows that it belongs to the family of leucine-rich repeat (LRR) proteins. It is expressed at high levels in certain zones of cartilage and also has been detected in bone, tendon, bone marrow, and chondrosarcoma cells. CHAD promotes attachment of chondrocytes, fibroblasts, and osteoblasts, mediated via the integrin alpha2beta1. It has also been shown to bind to collagen type II and both collagen type II and chondroadherin interact with integrin alpha2beta1. Besides, CHAD may play an important role in the regulation of chondrocyte growth and proliferation.

Publications

Species	Application	Title
human	WB	Reprod Sci; NFAT Overexpression Correlates with CA72-4 and Poor Prognosis of Ovarian Clear-Cell Carcinoma Subtype.; Authors - Bing Xin

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