Anticorps Polyclonal de lapin anti-Cadherin-17

Cadherin-17 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 24339-1-AP

Synonymes

CDH17, Intestinal peptide-associated transporter HPT-1, HPT1, HPT 1, CDH16

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Galerie de données de validation

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  WB analysis using 24339-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24339-1-AP (Cadherin-17 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 24339-1-AP

  Immunohistochemical analysis of paraffin-embedded colon cancer slide using 24339-1-AP (Cadherin-17 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 24339-1-AP

  Immunohistochemical analysis of paraffin-embedded colon cancer slide using 24339-1-AP (Cadherin-17 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 24339-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24339-1-AP (Cadherin-17 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 24339-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24339-1-AP (Cadherin-17 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse colon using 24339-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 24339-1-AP (Cadherin-17 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 24339-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 24339-1-AP (Cadherin-17 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 24339-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24339-1-AP (Cadherin-17 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach cancer using 24339-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24339-1-AP (Cadherin-17 antibody at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu de côlon de souris, tissu d'intestin grêle de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer de l'estomac humain, tissu de côlon de souris, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

24339-1-AP cible Cadherin-17 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cadherin-17 Protéine recombinante Ag19488
• Nom complet: cadherin 17, LI cadherin (liver-intestine)
• Masse moléculaire calculée: 832 aa, 92 kDa
• Poids moléculaire observé: 120 kDa
• Numéro d’acquisition GenBank: BC113464
• Symbole du gène: Cadherin-17
• Identification du gène (NCBI): 1015
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cadherin-17, also known as LI-cadherin, is a member of the cadherin superfamily. Cadherins are transmembrane proteins that mediates cell-cell adhesion, plays important roles during embryogenesis, and is crucial for tissue morphogenesis and maintenance. Cadherin-17 is a component of the gastrointestinal tract and pancreatic ducts and is involved in intestinal peptide transport. It may also play a role in the morphological organization of liver and intestine.

Publications

Species	Application	Title
human	WB	Elife; HNF1A is a novel oncogene that regulates human pancreatic cancer stem cell properties.; Authors - Ethan V Abel
human	IHC	Jpn J Clin Oncol; Establishing metastatic patient-derived xenograft model for colorectal cancer.; Authors - Yanmei Zhang
human	WB	J Biol Chem; Host cell membrane proteins located near SARS-CoV-2 spike protein attachment sites are identified using proximity labeling and proteomic analysis; Authors - Norihiro Kotani
human	WB	Int J Mol Sci; A Regulator Role for the ATP-Binding Cassette Subfamily C Member 6 Transporter in HepG2 Cells: Effect on the Dynamics of Cell-Cell and Cell-Matrix Interactions; Authors - Ilenia Matera
human	WB,IHC,IF,IP	J Exp Clin Cancer Res; A complex of cadherin 17 with desmocollin 1 and p120-catenin regulates colorectal cancer migration and invasion according to the cell phenotype; Authors - Rubén A Bartolomé ; KD Validated

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