CDX2 Monoclonal antibody

• Featured Product
• KD/KO Validated

CDX2 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

13

CloneNo.

5A4E6

Cat no : 60243-1-Ig

Synonyms

caudal type homeobox 2, Caudal type homeobox protein 2, CDX 3, CDX2, CDX-2, CDX3, Homeobox protein CDX 2

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Validation Data Gallery

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  WB analysis of COLO 320 using 60243-1-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 60243-1-Ig (CDX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of SW 1990 using 60243-1-Ig

  SW 1990 cells were subjected to SDS PAGE followed by western blot with 60243-1-Ig (CDX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig colon using 60243-1-Ig

  pig colon tissue were subjected to SDS PAGE followed by western blot with 60243-1-Ig (CDX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-29 using 60243-1-Ig

  HT-29 cells were subjected to SDS PAGE followed by western blot with 60243-1-Ig (CDX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of Caco-2 using 60243-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using CDX2 antibody (60243-1-Ig, Clone: 5A4E6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: COLO 320 cells, HT-29 cells, SW 1990 cells, pig colon tissue
• Positive IF/ICC detected in: Caco-2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 7 publications below

Product Information

The immunogen of 60243-1-Ig is CDX2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, pig
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CDX2 fusion protein Ag17644
• Full Name: caudal type homeobox 2
• Calculated Molecular Weight: 313 aa, 34 kDa
• Observed Molecular Weight: 33-40 kDa
• GenBank Accession Number: BC014461
• Gene Symbol: CDX2
• Gene ID (NCBI): 1045
• RRID: AB_2881365
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDX2, also named as Homeobox protein CDX-2, is a 313 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the Caudal homeobox family. CDX2 localizes in the nucleus and is involved in the transcriptional regulation of multiple genes expression in the intestinal epithelium. The relative expression of CDX1 to CDX2 protein may be important in the anterior to posterior patterning of the intestinal epithelium and in defining patterns of proliferation and differentiation along the crypt-villus axis. Both Cdx1 and Cdx2 genes must be expressed to reduce tumorigenic potential, to increase sensitivity to apoptosis, and to reduce cell migration, suggesting that the two genes control the normal phenotype by independent pathways.

Publications

Species	Application	Title
	WB	Aging (Albany NY); The role of CDX2 in renal tubular lesions during diabetic kidney disease.; Authors - Huiming Liu ; KD Validated
human	WB,IF	Mediators Inflamm; Vitronectin Destroyed Intestinal Epithelial Cell Differentiation through Activation of PDE4-Mediated Ferroptosis in Inflammatory Bowel Disease; Authors - Wenxu Pan
	IF	J Reprod Immunol; Poly(I:C) exposure during in vitro fertilization disrupts first cleavage of mouse embryos and subsequent blastocyst development.; Authors - Zhicheng Wang
human	WB	Chem Biol Interact; 6-Formylindolo(3,2-b)carbazole induced aryl hydrocarbon receptor activation prevents intestinal barrier dysfunction through regulation of claudin-2 expression.; Authors - Yuanhang Ma ; KD Validated
	IHC	World J Gastrointest Oncol; Xiaojianzhong decoction prevents gastric precancerous lesions in rats by inhibiting autophagy and glycolysis in gastric mucosal cells; Authors - Jia-Xiang Zhang
	IF	Mol Reprod Dev; Ratio of the zygote cytoplasm to the paternal genome affects the reprogramming and developmental efficiency of androgenetic embryos.; Authors - Chen Liao