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Anticorps Polyclonal de lapin anti-Cadherin-13

Cadherin-13 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 12618-1-AP

Synonymes

Cadherin 13, Cadherin-13, CDH13, CDHH, H cadherin, H-cadherin, Heart cadherin, P105, T cad, T cadherin, T-cadherin, Truncated cadherin

Galerie de données de validation

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  • Western Blot (WB) analysis of mouse heart tissue using Cadherin-13 Polyclonal antibody (12618-1-AP)

    WB analysis of mouse heart using 12618-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 12618-1-AP (Cadherin-13 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using Cadherin-13 Polyclonal antibody (12618-1-AP)

    WB analysis of L02 using 12618-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 12618-1-AP (Cadherin-13 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using Cadherin-13 Polyclonal antibody (12618-1-AP)

    WB analysis of human heart using 12618-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 12618-1-AP (Cadherin-13 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse heart tissue using Cadherin-13 Polyclonal antibody (12618-1-AP)

    IP experiment of mouse heart using 12618-1-AP

    IP result of anti-Cadherin-13 (IP:12618-1-AP, 4ug; Detection:12618-1-AP 1:700) with mouse heart tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using Cadherin-13 Polyclonal antibody (12618-1-AP)

    IHC staining of human endometrial cancer using 12618-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 12618-1-AP (Cadherin-13 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using Cadherin-13 Polyclonal antibody (12618-1-AP)

    IHC staining of human heart using 12618-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12618-1-AP (Cadherin-13 antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using Cadherin-13 Polyclonal antibody (12618-1-AP)

    IHC staining of human heart using 12618-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12618-1-AP (Cadherin-13 antibody at dilution of 1:50 (under 40x lens).

Applications testées

Résultats positifs en WBtissu cardiaque de souris, cellules L02, tissu cardiaque humain
Résultats positifs en IPtissu cardiaque de souris
Résultats positifs en IHCtissu de cancer de l'endomètre humain, tissu cardiaque humain
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochimie (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

12618-1-AP cible Cadherin-13 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène Cadherin-13 Protéine recombinante Ag3295
Nom complet cadherin 13, H-cadherin (heart)
Masse moléculaire calculée 713 aa, 78 kDa
Poids moléculaire observé 105 kDa
Numéro d’acquisition GenBankBC030653
Symbole du gène Cadherin-13
Identification du gène (NCBI) 1012
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. Cadherin-13 (CDH13, also known as H-cadherin or T-cadherin) is an atypical member of cadherin family, lacking transmembrane and cytosolic domains and anchored to the exterior surface of the plasma membrane via a GPI anchor. CDH13 is thought to affect cellular behavior largely through its signaling properties. It is highly expressed in heart, and in the CNS, CDH13 is expressed in cerebral cortex, medulla, hippocampus, amygdala, thalamus and substantia nigra. It is often down-regulated in cancerous cells. The CDH13 cDNA encodes a 713-amino acid protein, which has a calculated molecular weight of 78 kDa. Studies have detected variable bands on Western blots, including 105 kDa (mature form), 130 kDa (precursor), and 45 kDa, which may be a post-translational processing variant of CDH13 (PMID: 19399994).

Protocole

Product Specific Protocols
WB protocol for Cadherin-13 antibody 12618-1-APDownload protocol
IHC protocol for Cadherin-13 antibody 12618-1-APDownload protocol
IP protocol for Cadherin-13 antibody 12618-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols