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N-cadherin Monoclonal antibody
N-cadherin Monoclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, pig, mouse, rat, rabbit and More (1)
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
174
CloneNo.
1D8B3
Cat no : 66219-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | rabbit brain tissue, pig brain tissue, human heart tissue, MKN-45 cells, HEK-293 cells, PC-3 cells, mouse brain tissue, rat brain tissue, C2C12 cells, C6 cells, Saos-2 cells, SGC-7901 cells, ROS1728 cells, NIH/3T3 cells |
Positive IHC detected in | mouse heart tissue, mouse brain tissue, human malignant melanoma tissue, human ovary tumor tissue, rat heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse heart tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:7500-1:30000 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 148 publications below |
IHC | See 17 publications below |
IF | See 28 publications below |
Product Information
66219-1-Ig targets N-cadherin in WB, IHC, IF-P, ELISA applications and shows reactivity with human, pig, mouse, rat, rabbit samples.
Tested Reactivity | human, pig, mouse, rat, rabbit |
Cited Reactivity | human, mouse, rat, pig, monkey |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | N-cadherin fusion protein Ag4996 |
Full Name | cadherin 2, type 1, N-cadherin (neuronal) |
Calculated Molecular Weight | 906 aa, 100 kDa |
Observed Molecular Weight | 130 kDa |
GenBank Accession Number | BC036470 |
Gene Symbol | N-cadherin |
Gene ID (NCBI) | 1000 |
RRID | AB_2881610 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a 130-kDa transmembrane protein and a classical member of the cadherin superfamily which also include E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes (PMID: 11282032; 9508779; 8125202). N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity (PMID: 23321619). The N-cadherin ectodomain can be cleaved, leading to the generation of a 90 kD N-terminal fragment (PMID: 16998833; 17028923).
Protocols
Product Specific Protocols | |
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WB protocol for N-cadherin antibody 66219-1-Ig | Download protocol |
IHC protocol for N-cadherin antibody 66219-1-Ig | Download protocol |
IF protocol for N-cadherin antibody 66219-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Stem Cell Long-term engraftment and maturation of autologous iPSC-derived cardiomyocytes in two rhesus macaques | ||
Mol Cancer YTHDF2 mediates the mRNA degradation of the tumor suppressors to induce AKT phosphorylation in N6-methyladenosine-dependent way in prostate cancer. | ||
J Pineal Res Melatonin and verteporfin synergistically suppress the growth and stemness of head and neck squamous cell carcinoma through the regulation of mitochondrial dynamics | ||
Aging Cell Advanced Maternal Age-associated SIRT1 Deficiency Compromises Trophoblast Epithelial-Mesenchymal Transition through an Increase in Vimentin Acetylation. | ||
Mater Today Bio Ordered micropattern arrays fabricated by lung-derived dECM hydrogels for chemotherapeutic drug screening. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Davide (Verified Customer) (06-13-2023) | The antibody didn't produce any signal in 3d primary human cholangiocyte organoids. I would recommend either to try other antibody concentration or to try another Ab. It was tried on a cell condition which was recently reported to stimulate the CDH2 protein expression. However it should also be present in control condition at low levels.
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FH Udesh (Verified Customer) (10-13-2022) | Used the Ab at 1:2000 dilution in 5% NFDM and observed a band at 140 kD. Blocked in 5% NFDM for 1H at RT.
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