Anticorps Monoclonal anti-CD71

• Phare
• Validé par KD/KO

CD71 Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, IP, IHC, IF, FC, Blocking assay, ELISA

Conjugaison

Non conjugué

Publications

15

CloneNo.

3C11F11

N° de cat : 66180-1-Ig

Synonymes

CD71, p90, sTfR, T9, TFR, TFR1, TFRC, TR, Transferrin receptor protein 1, TRFR

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Galerie de données de validation

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  WB analysis of Raji using 66180-1-Ig

  Untreated and PNGase F-treated lysates of Raji cells and HeLa cells were subjected to SDS PAGE followed by western blot with 66180-1-Ig (CD71 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

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  WB analysis using 66180-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66180-1-Ig (CD71 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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  WB analysis of TF-1 using 66180-1-Ig

  TF-1 cells were subjected to SDS PAGE followed by western blot with 66180-1-Ig (CD71 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 66180-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 66180-1-Ig (CD71 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 66180-1-Ig

  IP Result of anti-CD71 (IP:66180-1-Ig, 5ug; Detection:66180-1-Ig 1:1000) with HeLa cells lysate 2400ug.

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  IHC staining of human placenta using 66180-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66180-1-Ig (CD71 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 66180-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66180-1-Ig (CD71 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66180-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66180-1-Ig (CD71 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66180-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66180-1-Ig (CD71 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human breast cancer using 66180-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CD71 antibody (66180-1-Ig, Clone: 3C11F11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of MCF-7 using 66180-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CD71 antibody (66180-1-Ig, Clone: 3C11F11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of Raji using 66180-1-Ig

  1X10^6 Raji cells were stained with 0.2ug CD71 antibody (66180-1-Ig, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). FITC-Goat anti-mouse IgG with dilution 1:100.

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Applications testées

• Résultats positifs en WB: cellules U-251, cellules A431, cellules A549, cellules HeLa, cellules HepG2, cellules K-562, cellules Raji, cellules TF-1, cellules U2OS
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu placentaire humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu de cancer du sein humain, cellules MCF-7
• Résultats positifs en cytométrie: cellules Raji

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 13 publications below
• IF: See 3 publications below

Informations sur le produit

66180-1-Ig cible CD71 dans les applications de WB, IP, IHC, IF, FC, Blocking assay, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD71 Protéine recombinante Ag21612
• Nom complet: transferrin receptor (p90, CD71)
• Masse moléculaire calculée: 85 kDa
• Poids moléculaire observé: 90 kDa
• Numéro d’acquisition GenBank: BC001188
• Symbole du gène: TFRC
• Identification du gène (NCBI): 7037
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex which enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.

Publications

Species	Application	Title
human	IF	Chem Eng J; Functionalized biological metal–organic framework with nanosized coronal structure and hierarchical wrapping pattern for enhanced targeting therapy; Authors - Huafeng Wang
human	WB	Cell Death Dis; Super-enhancer-driven MLX mediates redox balance maintenance via SLC7A11 in osteosarcoma; Authors - Weitang Guo
human,mouse	WB	Free Radic Biol Med; Targeting the Na+/K+ ATPase DR-region with DR-Ab improves doxorubicin-induced cardiotoxicity; Authors - Bin Leng
human	WB,Blocking assay	Bioeng Transl Med; Multistage targeting and dual inhibiting strategies based on bioengineered tumor matrix microenvironment-mediated protein nanocages for enhancing cancer biotherapy.; Authors - Fabiao Hu
human	WB	PLoS Genet; SNX-3 mediates retromer-independent tubular endosomal recycling by opposing EEA-1-facilitated trafficking.; Authors - Yangli Tian
human	IF	J Biol Chem; Rab11-mediated post-Golgi transport of the sialyltransferase ST3GAL4 suggests a new mechanism for regulating glycosylation.; Authors - Masato Kitano

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