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  • KD/KO Validated

CD71 Polyclonal antibody

CD71 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human and More (4)


WB, IP, IF, IHC, ELISA, Cell treatment



Cat no : 10084-2-AP


CD71, p90, sTfR, T9, TFR, TFR1, TFRC, TR, Transferrin receptor protein 1, TRFR

Tested Applications

Positive WB detected inHT-1080 cells, HeLa cells, Jurkat cells, K-562 cells, human placenta tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10084-2-AP targets CD71 in WB, IP, IF, IHC, ELISA, Cell treatment applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, chicken, monkey, pig, duck
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD71 fusion protein Ag0123
Full Name transferrin receptor (p90, CD71)
Calculated Molecular Weight 85 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC001188
Gene Symbol TFRC
Gene ID (NCBI) 7037
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex that enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.


Product Specific Protocols
WB protocol for CD71 antibody 10084-2-APDownload protocol
IHC protocol for CD71 antibody 10084-2-APDownload protocol
IF protocol for CD71 antibody 10084-2-APDownload protocol
IP protocol for CD71 antibody 10084-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Res

The metabolite α-KG induces GSDMC-dependent pyroptosis through death receptor 6-activated caspase-8.

Authors - Jia-Yuan Zhang

Acta Neuropathol

Seizure-mediated iron accumulation and dysregulated iron metabolism after status epilepticus and in temporal lobe epilepsy.

Authors - Till S Zimmer

Cell Death Differ

Ferroptotic stress facilitates smooth muscle cell dedifferentiation in arterial remodelling by disrupting mitochondrial homeostasis

Authors - Qing-Xin Ji

Acta Biomater

Cyclosporine A-loaded apoferritin alleviates myocardial ischemia-reperfusion injury by simultaneously blocking ferroptosis and apoptosis of cardiomyocytes

Authors - Wenqiang Qian

Cancer Res

Intratumoral payload release influences the potency and bystander-killing effects of antibody-drug conjugates in preclinical models.

Authors - Fu Li

Cell Death Dis

Knockdown of SETD2 promotes erastin-induced ferroptosis in ccRCC

Authors - Wei Xue


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Nu (Verified Customer) (10-24-2023)

Although there is a band close to 100 kd, there are other even more stronger non-specific bands.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: postmortem human frontal cortex
CD71 Antibody Western Blot validation (1:1000 dilution) in postmortem human frontal cortex (Cat no:10084-2-AP)

Sara (Verified Customer) (10-25-2020)

Really good signal in WB, it worked great, even though I loaded 1-2 µg of total cell lysate in some lanes. Not that good for IF, the signal was a bit fuzzy and all over the cell.

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:1000 WB / 1:100 IF
  • Cell Tissue Type: B cells from mouse spleen
CD71 Antibody Western Blot,Immunofluorescence validation (1:1000 WB / 1:100 IF dilution) in B cells from mouse spleen (Cat no:10084-2-AP)