Anticorps Polyclonal de lapin anti-CD68
CD68 Polyclonal Antibody for IF, IHC, WB, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain et plus (2)
Applications
WB, IHC, IF, FC, ELISA
Conjugaison
Non conjugué
N° de cat : 25747-1-AP
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | cellules THP-1, cellules U-937 |
| Résultats positifs en IHC | tissu hépatique humain, tissu d'amygdalite humain il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
| Résultats positifs en IF | tissu d'amygdalite humain, tissu d'appendicite humain, tissu de côlon humain |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochimie (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| WB | See 11 publications below |
| IHC | See 38 publications below |
| IF | See 32 publications below |
| FC | See 2 publications below |
Informations sur le produit
25747-1-AP cible CD68 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain
| Réactivité | Humain |
| Réactivité citée | canin, Humain, porc |
| Hôte / Isotype | Lapin / IgG |
| Clonalité | Polyclonal |
| Type | Anticorps |
| Immunogène | CD68 Protéine recombinante Ag22815 |
| Nom complet | CD68 molecule |
| Masse moléculaire calculée | 37 kDa |
| Poids moléculaire observé | 60-70 kDa |
| Numéro d’acquisition GenBank | BC015557 |
| Symbole du gène | CD68 |
| Identification du gène (NCBI) | 968 |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par affinité contre l'antigène |
| Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages. The apparent molecular weight of CD68 is larger than calculated molecular weight due to post-translation modification.
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for CD68 antibody 25747-1-AP | Download protocol |
| IHC protocol for CD68 antibody 25747-1-AP | Download protocol |
| IF protocol for CD68 antibody 25747-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Metab Pharmacological inhibition of arachidonate 12-lipoxygenase ameliorates myocardial ischemia-reperfusion injury in multiple species. | ||
Theranostics Platelets promote CRC by activating the C5a/C5aR1 axis via PSGL-1/JNK/STAT1 signaling in tumor-associated macrophages | ||
Nat Commun The ubiquitin ligase ZNRF1 promotes caveolin-1 ubiquitination and degradation to modulate inflammation. | ||
Aging Cell Inhibition of DNA methyltransferase aberrations reinstates antioxidant aging suppressors and ameliorates renal aging. | ||
Aging Dis Sterol-resistant SCAP Overexpression in Vascular Smooth Muscle Cells Accelerates Atherosclerosis by Increasing Local Vascular Inflammation through Activation of the NLRP3 Inflammasome in Mice. |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Emma (Verified Customer) (11-29-2021) | Works well by IF on FFPE tissue with a Tris-EDTA antigen retrieval. Also works by IHC.
 |
FH Fabio Henrique (Verified Customer) (01-17-2019) | Figure 1. IF of CD68 (red) in human spleen. Formalin-fixed paraffin-embedded human spleen tissue was used as positive control to probe for CD68. Heat-induced antigen retrieval was performed in sodium citrate buffer pH 6.0 + Tween20 at 0.5%. Tissue incubated at 96*C for 20 min inside the buffer. Permeabilization was done with washes of TBSTritonX 0.25% for 3x 5min and blocking was done in TBS 10% Goat serum, 1% BSA, for 1 hr at RT. Anti-CD68 was used at 1:200 in blocking buffer, overnight incubation at 4*C. Secondary antibody AlexaFluor 488 was used at 1:500 for 1 hr at RT. Imaged using a Nikon A1 confocal microscope. Figure 2: CD68 (red) and CD206 (green) in human primary macrophages polarized to M2-phenotype, encapsulated in 3D hydrogel (hyaluronic acid and collagen type 1). Staining was performed as described above, except primary (CD68) was used at 1:100 and secondary antibodies were used at 1:500.
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