Anticorps Polyclonal de lapin anti-BBS7

BBS7 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 18961-1-AP

Synonymes

Bardet Biedl syndrome 7, BBS2 like protein 1, BBS2L1, BBS7

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Galerie de données de validation

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  WB analysis of human brain using 18961-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 18961-1-AP (BBS7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 18961-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 18961-1-AP (BBS7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 18961-1-AP

  IP result of anti-BBS7 (IP:18961-1-AP, 4ug; Detection:18961-1-AP 1:500) with mouse testis tissue lysate 4000ug.

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  IHC staining of human testis using 18961-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18961-1-AP (BBS7 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 18961-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18961-1-AP (BBS7 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 18961-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18961-1-AP (BBS7 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 18961-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18961-1-AP (BBS7 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain
• Résultats positifs en IP: tissu testiculaire de souris
• Résultats positifs en IHC: tissu testiculaire humain, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IHC: See 1 publications below
• IP: See 2 publications below

Informations sur le produit

18961-1-AP cible BBS7 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BBS7 Protéine recombinante Ag4601
• Nom complet: Bardet-Biedl syndrome 7
• Masse moléculaire calculée: 715 aa, 80 kDa
• Poids moléculaire observé: 75 kDa
• Numéro d’acquisition GenBank: BC032691
• Symbole du gène: BBS7
• Identification du gène (NCBI): 55212
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BBS7, also named as BBS2L1, is a component of the BBSome complex which is required for ciliogenesis but is dispensable for centriolar satellite function. BBS7 is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome.

Publications

Species	Application	Title
mouse	WB	Proc Natl Acad Sci U S A; Accumulation of non-outer segment proteins in the outer segment underlies photoreceptor degeneration in Bardet-Biedl syndrome.; Authors - Poppy Datta
mouse	WB	PLoS Genet; A novel protein LZTFL1 regulates ciliary trafficking of the BBSome and Smoothened.; Authors - Seo Seongjin S
human	WB	PLoS Genet; The Centriolar Satellite Protein AZI1 Interacts with BBS4 and Regulates Ciliary Trafficking of the BBSome.; Authors - Xitiz Chamling
mouse	WB	PLoS Genet; BBSome function is required for both the morphogenesis and maintenance of the photoreceptor outer segment.; Authors - Ying Hsu
mouse	IHC, IP, WB	Hum Mol Genet; Photoreceptor cilia, in contrast to primary cilia, grant entry to a partially assembled BBSome.; Authors - Ying Hsu
mouse	WB,IP	J Biol Chem; The myosin-tail homology domain of centrosomal protein 290 is essential for protein confinement between the inner and outer segments in photoreceptors.; Authors - Poppy Datta

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