BBS1 Polyclonal antibody

BBS1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Reviews (1)

Cat no : 21118-1-AP

Synonyms

Bardet Biedl syndrome 1, BBS1, BBS2 like protein 2, BBS2L2

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Validation Data Gallery

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  WB analysis of mouse kidney using 21118-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21118-1-AP (BBS1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 21118-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 21118-1-AP (BBS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 21118-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21118-1-AP (BBS1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 21118-1-AP

  IP result of anti-BBS1 (IP:21118-1-AP, 4ug; Detection:21118-1-AP 1:600) with mouse brain tissue lysate 4000ug.

• 

  IF Staining of hTERT-RPE1 cells using 21118-1-AP

  IF result (base of ciliary stain) of anti-BBS1 (21118-1-AP, 1:50) with with serum-starved hTERT-RPE1 cells by Dr. Moshe Kim.

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Tested Applications

• Positive WB detected in: mouse kidney tissue, mouse brain tissue, mouse heart tissue
• Positive IP detected in: mouse brain tissue
• Positive IF/ICC detected in: hTERT-RPE1 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

The immunogen of 21118-1-AP is BBS1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BBS1 fusion protein Ag15460
• Full Name: Bardet-Biedl syndrome 1
• Calculated Molecular Weight: 593 aa, 65 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC047642
• Gene Symbol: BBS1
• Gene ID (NCBI): 582
• RRID: AB_2878814
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	EMBO J; Rabl2 GTP hydrolysis licenses BBSome-mediated export to fine-tune ciliary signaling.; Authors - Shichao Duan
human	IF	J Biol Chem; Non-redundant roles of DIAPHs in primary ciliogenesis.; Authors - Oliva Palander

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jerome (Verified Customer) (05-07-2019)

Nuclear staining, and perinuclear staining that looks like a Golgi

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 100
• Cell Tissue Type: Hela Cells

BBS1 Antibody Immunofluorescence, validation (100 dilution) in Hela Cells (Cat no:21118-1-AP)