|Positive WB detected in||HEK-293 cells, mouse kidney tissue|
|Positive IHC detected in||human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||hTERT-RPE1 cells|
|Western Blot (WB)||WB : 1:500-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
12766-1-AP targets BBS4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||BBS4 fusion protein Ag3430|
|Full Name||Bardet-Biedl syndrome 4|
|Calculated molecular weight||519 aa, 58 kDa|
|Observed molecular weight||50-60 kDa|
|GenBank accession number||BC027624|
|Gene ID (NCBI)||585|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Coupling bimolecular PARylation biosensors with genetic screens to identify PARylation targets.
Loss of Bardet-Biedl syndrome proteins causes synaptic aberrations in principal neurons.
The CEP19-RABL2 GTPase Complex Binds IFT-B to Initiate Intraflagellar Transport at the Ciliary Base.
The ciliary protein RPGRIP1L governs autophagy independently of its proteasome-regulating function at the ciliary base in mouse embryonic fibroblasts.
Aurora Kinase A proximity map reveals centriolar satellites as regulators of its ciliary function.
Islet vascularization is regulated by primary endothelial cilia via VEGF-A-dependent signaling.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Christine (Verified Customer) (01-27-2023)
Detect 1 band above 47 kDa marker (expected size of BBS4 is 50-60 kDa), but also a much stronger band just below the 20 kDa marker