BBS5 Polyclonal antibody

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  WB analysis of mouse retina using 14569-1-AP

  mouse retina tissue were subjected to SDS PAGE followed by western blot with 14569-1-AP (BBS5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 14569-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 14569-1-AP (BBS5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 14569-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 14569-1-AP (BBS5 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse eye using 14569-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 14569-1-AP (BBS5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 14569-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14569-1-AP (BBS5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 14569-1-AP

  IP result of anti-BBS5 (IP:14569-1-AP, 4ug; Detection:14569-1-AP 1:1500) with mouse testis tissue lysate 1120 ug.

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  IHC staining of human testis using 14569-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14569-1-AP (BBS5 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 14569-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14569-1-AP (BBS5 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human stomach using 14569-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 14569-1-AP (BBS5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 14569-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 14569-1-AP (BBS5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 14569-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14569-1-AP (BBS5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 14569-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14569-1-AP (BBS5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 14569-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14569-1-AP (BBS5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 14569-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14569-1-AP (BBS5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 14569-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14569-1-AP (BBS5 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 14569-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14569-1-AP (BBS5 Antibody) at dilution of 1:200 (under 40x lens).

• Featured Product
• KD/KO Validated

BBS5 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 14569-1-AP

Publications(27)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

Bardet-Biedl syndrome 5 protein, Bardet Biedl syndrome 5

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Tested Applications

• Positive WB detected in: mouse retina tissue, mouse testis tissue, human testis tissue, rat testis tissue, mouse eye tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human testis tissue, human kidney tissue, human stomach tissue, mouse kidney tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 14 publications below
• IHC: See 1 publications below
• IF: See 19 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

14569-1-AP targets BBS5 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BBS5 fusion protein Ag6153
• Full Name: Bardet-Biedl syndrome 5
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC044593
• Gene Symbol: BBS5
• Gene ID (NCBI): 129880
• RRID: AB_10597551
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8N3I7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BBS5 encodes a protein that has been directly linked to Bardet-Biedl syndrome. Bardet-Biedl syndrome (BBS) is an autosomal recessive condition characterised by rod-cone dystrophy, postaxial polydactyly, central obesity, mental retardation, hypogonadism, and renal dysfunction. Other associated clinical findings in BBS patients include diabetes, hypertension and congenital heart defects. BBS expression varies both within and between families and diagnosis is often difficult. Experimentation in non-human eukaryotes suggests that BBS5 is expressed in ciliated cells and that it is required for the formation of cilia. Alternate transcriptional splice variants have been observed but have not been fully characterized.

Publications

Species	Application	Title
human	IF	Nat Genet; A transition zone complex regulates mammalian ciliogenesis and ciliary membrane composition.; Authors - Garcia-Gonzalo Francesc R FR
human	IF	Cell; The conserved Bardet-Biedl syndrome proteins assemble a coat that traffics membrane proteins to cilia.; Authors - Jin Hua H
human	WB	Nat Cell Biol; Systematic proteomics of the VCP-UBXD adaptor network identifies a role for UBXN10 in regulating ciliogenesis.; Authors - Malavika Raman
mouse	WB,IF	Dev Cell; IFT27 Links the BBSome to IFT for Maintenance of the Ciliary Signaling Compartment.; Authors - Thibaut Eguether
mouse	IF	Dev Cell; The Intraflagellar Transport Protein IFT27 Promotes BBSome Exit from Cilia through the GTPase ARL6/BBS3.; Authors - Gerald M Liew
rat	IF	Nat Struct Mol Biol; Structural basis for membrane targeting of the BBSome by ARL6.; Authors - André Mourão