BBS5 Polyclonal antibody

• Featured Product
• KD/KO Validated

BBS5 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Publications

22

Cat no : 14569-1-AP

Synonyms

Bardet Biedl syndrome 5, BBS5

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Validation Data Gallery

• 

  WB analysis of mouse retina using 14569-1-AP

  mouse retina tissue were subjected to SDS PAGE followed by western blot with 14569-1-AP (BBS5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse eye using 14569-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 14569-1-AP (BBS5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 14569-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 14569-1-AP (BBS5 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 14569-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14569-1-AP (BBS5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 14569-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 14569-1-AP (BBS5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 14569-1-AP

  IP result of anti-BBS5 (IP:14569-1-AP, 4ug; Detection:14569-1-AP 1:600) with mouse testis tissue lysate 4000 ug.

• 

  IHC staining of human testis using 14569-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14569-1-AP (BBS5 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 14569-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14569-1-AP (BBS5 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse testis using 14569-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14569-1-AP (BBS5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 14569-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14569-1-AP (BBS5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 14569-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14569-1-AP (BBS5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 14569-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14569-1-AP (BBS5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 14569-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14569-1-AP (BBS5 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 14569-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14569-1-AP (BBS5 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human stomach using 14569-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 14569-1-AP (BBS5 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach using 14569-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 14569-1-AP (BBS5 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse retina tissue, mouse testis tissue, human testis tissue, rat testis tissue, mouse eye tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human testis tissue, mouse kidney tissue, human stomach tissue, human kidney tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 17 publications below

Product Information

14569-1-AP targets BBS5 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BBS5 fusion protein Ag6153
• Full Name: Bardet-Biedl syndrome 5
• Calculated molecular weight: 39 kDa
• Observed molecular weight: 39 kDa
• GenBank accession number: BC044593
• Gene symbol: BBS5
• Gene ID (NCBI): 129880
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

BBS5 encodes a protein that has been directly linked to Bardet-Biedl syndrome. Bardet-Biedl syndrome (BBS) is an autosomal recessive condition characterised by rod-cone dystrophy, postaxial polydactyly, central obesity, mental retardation, hypogonadism, and renal dysfunction. Other associated clinical findings in BBS patients include diabetes, hypertension and congenital heart defects. BBS expression varies both within and between families and diagnosis is often difficult. Experimentation in non-human eukaryotes suggests that BBS5 is expressed in ciliated cells and that it is required for the formation of cilia. Alternate transcriptional splice variants have been observed but have not been fully characterized.

Publications

Species	Application	Title
mouse	WB,IF	PLoS Biol; Loss of Bardet-Biedl syndrome proteins causes synaptic aberrations in principal neurons.; Authors - Naila Haq ; KO Validated
mouse	IF	Cell Rep; FBF1 deficiency promotes beiging and healthy expansion of white adipose tissue.; Authors - Yingyi Zhang
human	IF	Nephrology (Carlton); Visualizing renal primary cilia.; Authors - James A Deane
mouse	WB	Invest Ophthalmol Vis Sci; Essential Role of the Chaperonin CCT in Rod Outer Segment Biogenesis.; Authors - Satyabrata Sinha
human	IF, WB	J Biol Chem; The BBSome assembly is spatially controlled by BBS1 and BBS4 in human cells.; Authors - Avishek Prasai
human	WB,IF	J Biol Chem; DAZ interacting protein 1 (Dzip1) phosphorylation by Polo-like kinase 1 (Plk1) regulates the centriolar satellites localization of the BBSome during the cell cycle.; Authors - Boyan Zhang

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