• Phare
  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-AXL

AXL Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugaison

Non conjugué

N° de cat : 13196-1-AP

Synonymes

AXL, AXL oncogene, AXL receptor tyrosine kinase, JTK11, UFO



Applications testées

Résultats positifs en WBcellules DU 145, cellules HeLa, cellules HepG2, cellules HT-1080, tissu hépatique humain
Résultats positifs en IPcellules HeLa,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

13196-1-AP cible AXL dans les applications de WB, IP, IF, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Réactivité citéerat, Humain
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène AXL Protéine recombinante Ag3898
Nom complet AXL receptor tyrosine kinase
Masse moléculaire calculée 894 aa, 98 kDa
Poids moléculaire observé 140 kDa
Numéro d’acquisition GenBankBC032229
Symbole du gène AXL
Identification du gène (NCBI) 558
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Axl, also known as Ufo or Ark, is a receptor tyrosine kinase of the TAM (Tyro3, Axl and Mer) family. Axl is a single-pass type I membrane protein containing two fibronectin type-III domains, two Ig-like C2-type domains and one protein kinase domain. Axl mRNA is widely expressed with highest levels in the heart and skeletal muscle. Gas6 has been identified as a ligand for human Axl. Gas6/Axl signaling plays a role in cell survival, cell proliferation, migration and differentiation. It has been reported that 110-120 kDa non-glycosylated AXL protein can be detected(PMID: 31171001). Endogenous Axl is auto-phosphorylated and reacted with a tyrosine-phosphorylated protein of 140 kDa(PMID: 12730092).

Protocole

Product Specific Protocols
WB protocol for AXL antibody 13196-1-APDownload protocol
IP protocol for AXL antibody 13196-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Cancer

Therapeutic Activity of DCC-2036, a Novel Tyrosine Kinase Inhibitor, against Triple-Negative Breast Cancer Patient-Derived Xenografts by Targeting AXL/MET.

Authors - Yingying Shen
ratWB

Int J Mol Sci

Embryonic-Derived Myb- Macrophages Enhance Bacterial Clearance and Improve Survival in Rat Sepsis.

Authors - Mirjana Jerkic
humanWB

Int J Mol Sci

Upregulation of miR-34a-5p, miR-20a-3p and miR-29a-3p by Onconase in A375 Melanoma Cells Correlates with the Downregulation of Specific Onco-Proteins.

Authors - Elisa De Tomi
FC

Bioorg Chem

Non-volatile acylphloroglucinol components from Eucalyptus robusta inhibit Zika virus by impairing RdRp activity of NS5.

Authors - Zhai-Wen Yao
humanWB, IHC,IF

Cancer Gene Ther

Targeting LRIG2 overcomes resistance to EGFR inhibitor in glioblastoma by modulating GAS6/AXL/SRC signaling.

Authors - Minhai Dong
humanWB

iScience

SUFU suppresses ferroptosis sensitivity in breast cancer cells via Hippo/YAP pathway.

Authors - Kun Fang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Marta (Verified Customer) (05-30-2022)

The antibody works well. Non-specific bands are not detected.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: A375 melanoma cell line