Anticorps Polyclonal de lapin anti-Ataxin 2

• Phare
• Validé par KD/KO

Ataxin 2 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

32

Avis (1)

N° de cat : 21776-1-AP

Synonymes

ATXN2, SCA2, ATXN 2, ATX2, Ataxin-2

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Galerie de données de validation

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  WB analysis using 21776-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21776-1-AP (Ataxin 2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 21776-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21776-1-AP (Ataxin 2 antibody) at dilution of 1:9000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 21776-1-AP

  IP result of anti-Ataxin 2 (IP:21776-1-AP, 4ug; Detection:21776-1-AP 1:1000) with HEK-293 cells lysate 1200ug.

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  IHC staining of mouse brain using 21776-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21776-1-AP (Ataxin 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 21776-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21776-1-AP (Ataxin 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 21776-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Ataxin 2 antibody (21776-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 21776-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Ataxin 2 antibody (21776-1-AP) at dilution of 1:900 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules Jurkat, cellules Neuro-2a
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris,
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:450-1:1800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 18 publications below
• IHC: See 3 publications below
• IF: See 17 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below

Informations sur le produit

21776-1-AP cible Ataxin 2 dans les applications de WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Ataxin 2 Protéine recombinante Ag16470
• Nom complet: ataxin 2
• Masse moléculaire calculée: 1313 aa, 140 kDa
• Poids moléculaire observé: 140-150 kDa
• Numéro d’acquisition GenBank: BC114546
• Symbole du gène: ATXN2
• Identification du gène (NCBI): 6311
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Background

 ATXN2 (Ataxin-2) is a eukaryotic RNA‐binding protein that is conserved across species. It contains two LSm domains (RNA binding), a PAM2 motif (association with poly(A)-binding protein), and an N-terminal polyglutamine tract (PMID: 25027299). Ataxin-2 plays crucial roles at different stages of the regulation of posttranslational gene expression. ATXN2 is also directly implicated in the regulation of neural function by influencing specific molecular and cellular pathways. Genetic expansion of a poly‐glutamine tract in human ATXN2 has been linked to several neurodegenerative diseases, where it most likely acts through gain‐of‐function effects.

 What is the molecular weight of ATXN2? 

 The molecular weight varies from 12 to 140 kDa, depending on the isoform.

 What are the isoforms of ATXN2?

 ATXN2 has many isoforms, which differ significantly in length and domain composition.

 What is the subcellular localization of ATXN2?

 According to siRNA-based experiments, it is localized in the cytoplasmic compartment of normal cells, where it mostly associates with the Golgi apparatus and stress granules (PMID: 22508507).

 What is the tissue specificity of ATXN2?

 ATXN2 protein has been detected in various tissues; however, it is most highly expressed and studied in CNS. Significant amounts of this protein can also be detected in the liver and gallbladder.

 What is the molecular function of ATXN2?

 Ataxin-2 is involved in regulating various steps of mRNA translation, including poly‐A tailing, RNA stabilization, microRNA‐dependent gene silencing, and translational activation. All those functions are linked to its interactions with the poly(A)-binding protein. Ataxin-2 is involved in the formation of stress granules and P-bodies. Furthermore, genetic models of ATXN2 loss‐of‐function have underlined the importance of ATXN2 in mTOR signaling and cellular metabolism, which are all crucial for neural homeostasis (PMID: 29869836).

 What is ATXN2's involvement in disease?

 The N-terminal region of ATXN2 normally contains a polyQ stretch of 14-31 residues that can be expanded in the pathogenic state to 32-200 residues. Intermediate length expansions of this tract increase susceptibility to amyotrophic lateral sclerosis (ALS or Lou Gehrig's disease) (PMID: 20740007). Long expansions of this tract result in spinocerebellar ataxia-2 (SCA2), an autosomal dominantly inherited, neurodegenerative disorder (PMID: 29427103). Genome-wide association studies indicate that loss-of-function mutations in this gene are associated with susceptibility to type I diabetes, obesity, and hypertension.

Publications

Species	Application	Title
human	WB	Cell; Diverse CMT2 neuropathies are linked to aberrant G3BP interactions in stress granules; Authors - Qinqin Cui
mouse	IF,WB	Cell; Circadian clocks are modulated by compartmentalized oscillating translation; Authors - Yanrong Zhuang
mouse	WB	Mol Cell; Autism-Misregulated eIF4G Microexons Control Synaptic Translation and Higher Order Cognitive Functions.; Authors - Thomas Gonatopoulos-Pournatzis
human	WB,IF	Neuron; Small-Molecule Modulation of TDP-43 Recruitment to Stress Granules Prevents Persistent TDP-43 Accumulation in ALS/FTD.; Authors - Mark Y Fang
human	IF	Nat Commun; NUP62 localizes to ALS/FTLD pathological assemblies and contributes to TDP-43 insolubility.; Authors - Amanda M Gleixner
human,rat	WB, CoIP	Diabetologia; Saturated fatty acids entrap PDX1 in stress granules and impede islet beta cell function.; Authors - Mu Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tatyana (Verified Customer) (01-21-2023)

Very good clean signal in ICC. Labels cytoplasmic stress granules well. Antibody was incubated 2 h at RT at 1:100o dilution and could also be reused.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human / SH-SY5Y cells

Ataxin 2 Antibody Immunofluorescence validation (1:1000 dilution) in Human / SH-SY5Y cells (Cat no:21776-1-AP)

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