Tested Applications
| Positive WB detected in | Jurkat cells, HeLa cells, MKN-45 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human tonsillitis tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24822-1-AP targets ATXN2L in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20581 Product name: Recombinant human ATXN2L protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 712-1062 aa of BC082760 Sequence: SYIPQIHMGPAVQAPQMYPYPVSNSVPGQQGKYRGAKGSLPPQRSDQHQPASAPPMMQAAAAAGPPLVAATPYSSYIPYNPQQFPGQPAMMQPMAHYPSQPVFAPMLQSNPRMLTSGSHPQAIVSSSTPQYPSAEQPTPQALYATVHQSYPHHATQLHAHQPQPATTPTGSQPQSQHAAPSPVQHQAGQAPHLGSGQPQQNLYHPGALTGTPPSLPPGPSAQSPQSSFPQPAAVYAIHHQQLPHGFTNMAHVTQAHVQTGITAAPPPHPGAPHPPQVMLLHPPQSHGGPPQGAVPQSGVPALSASTPSPYPYIGHPQGEQPGQAPGFPGGADDRILQSHPSQQLPFHPPGN Predict reactive species |
| Full Name | ataxin 2-like |
| Calculated Molecular Weight | 1062 aa, 112 kDa |
| Observed Molecular Weight | ~140 kDa |
| GenBank Accession Number | BC082760 |
| Gene Symbol | ATXN2L |
| Gene ID (NCBI) | 11273 |
| RRID | AB_2879743 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8WWM7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ATXN2L is a paralog of Ataxin-2 (ATXN2) but without abnormal polyQ expended track, which is conserved in most of the ATXNs and drives the pathogenesis of neurodegeneration. It was reported that ATXN2L was widely expressed in immortalized cell lines, and ATXN2L-JAK2 fusion was found in CD4-positive T-cell lymphoma.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ATXN2L antibody 24822-1-AP | Download protocol |
| IHC protocol for ATXN2L antibody 24822-1-AP | Download protocol |
| IP protocol for ATXN2L antibody 24822-1-AP | Download protocol |
| WB protocol for ATXN2L antibody 24822-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-ATXN2L (Cat# 24822-1-AP) has 16 total citations across 14 journals, including Cell, Nature Communications, Neuron, Molecular Cell, Cell Reports, Cell Discovery, Cell Death & Disease, Neurobiology of Disease, Cell Genomics, Phytotherapy Research, Cells, Int J Mol Sci, ACS Chemical Biology, and Radiation Research. Associated research fields include stress granules, liquid-liquid phase separation, mRNA translation regulation, neurodegeneration (tauopathies), cancer (gastric/hepatocellular carcinoma), peripheral neuropathy, inflammasome activation, and apoptosis.
| Species | Application | Title |
|---|---|---|
Cell Diverse CMT2 neuropathies are linked to aberrant G3BP interactions in stress granules | ||
Nat Commun Ataxin-2-like promotes translation of nonpolyadenylated reovirus mRNA.
| ||
Nat Commun In situ secondary structure imaging of protein phase separation and aggregation by hyperspectral stimulated Raman scattering microscopy | ||
Neuron Increased G3BP2-Tau interaction in tauopathies is a natural defense against Tau aggregation | ||
Cell Discov RNA helicase DDX6 governs ASC speck formation in P-bodies and the transition to stress granules via phase separation during inflammasome activation. |
Reviews
What customers say
The only review for this product is from Sahil Kapoor (June 2019), who rated it 5 stars. He used it for Western Blot and stated that he had compared it with other options and found it works perfectly fine, adding that he would definitely buy it again if needed. Overall, the feedback is positive, highlighting reliability and satisfaction with the product's performance.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Sahil (Verified Customer) (06-21-2019) | I have checked others too and this works perfectly fine. If needed again would definitely buy it.
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